Abstract: Objective To investigate the influence of Gan-fu-kang on hepatic PDGF expression in rats with hepatic fibrosis. Methods The rats with liver fibrosis were made by subcutaneously injection of 10%CCl₄ and treated with Gan-fu-kang,a herbal medicine. Serum albumin,globulin,ALT and AST were measured. The PDGF expression in liver tissues were detected by immunohistochemistry staining. The collagenⅠmRNA were detected by RT-PCR. Results Compared with in model group,the levels of serum ALT,AST and globulin decreased,and albumin and the ratio of albumin to globulin increased （P<0.01） in Gan-fu-kang treatment group; the expression of PDGF in liver tissues in model group was obviously elevated than in Gan-fu-kang treatment group（P<0.01）. the collagenⅠmRNA level in model group increased than in control group and in treatment group（P<0.01）. Conclusion Gan-fu-kang has some effects on liver fibrosis by decreasing PDGF expression and inhibiting collagenⅠsynthesis.

Key words: Liver fibrosis, Hepatic stellate cell, Gan-fu-kang, PDGF, Collagen Ⅰ