Abstract: Objective To investigate the mechanisms by which Gan-fu-kang inhibiting Raf/ERK signal transduction pathway in rats with hepatic fibrosis. Methods The rats with liver fibrosi were made by subcutaneously injecting with 10%CCl4 and treated with Gan-fu-kang. Expression of Raf,ERK and collagen Ⅲ mRNA were detected by RT-PCR. The ERK expression in liver tissues were detected by immunohistochemistry staining. Results The levels of Raf,ERK and collagen Ⅲ mRNA in model group increased than in control group（P<0.01）;In the immunohistochemistry staining, the expression of ERK protein in model group was obviously elevated than in Gan-fu-kang treatment group. Conclusion Ganfukang has effective treatment on liver fibrosis and its treatment mechanisms probably involved in ERK-induced signal transduction pathway.

Key words: Liver fibrosis, Hepatic stellate cell, Gan-fu-kang, ERK, Signal transduction