Abstract: Objective To explore the protective effect and its mechanism of sevoflurane on sepsis-induced liver injury in rats. Methods Sixty SD rats were randomly divided into t sham operation,sepsis group and sevoflurane pretreatment group with 20 in each. In the sham operation group,the rats were only treated with open and closed abdominal suture,the sepsis model were established by cecal ligation perforation （CLP） and the animals in sevoflurane group were pretreated with sevoflurane anaesthesia before the establishment of CLP sepsis. The pathological changes of liver tissues were observed,and hepatic expression of lymphocyte function-associated antigen-1 （LFA-1） and macrophage-1 antigen （Mac-1） were detected by Western bloting. Results The pathological examination showed that the obvious inflammatory cell infiltration and hepatic cords arranged disorderly in model animals,while this kind of deformed morphology improved greatly in sevoflurane pretreatment group;serum AST,ALT and bilirubin levels in sepsis group were（156.8±14.7） U/L,（75.6±7.5） U/L and （4.5±0.6） μmol/L,while they decreased greatly in sevoflurane pretreatment group [（100.5±7.9） U/L,（61.5±10.1） U/L and （3.2±0.5） μmol/L,respectively,P<0.05];the Western bloting detection results demonstrated that the relative expression of hepatic LFA-1 and MAC-1 in sepsis animals were （1.2±0.2） and （1.0±0.2）,while they decreased greatly in sevoflurane pretreatment group[（0.7±0.2） and（0.6±0.1）,respectively,P<0.05]. Conclusion The application of sevoflurane for pretreatment anaesthesia might have a protective effect on sepsis-induced liver injury in rats,which is related to the down-regulation of hepatic LFA-1 and MAC-1 expression.

Key words: Sepsis, Sevoflurane, Anaesthesia, Lymphocyte function-associated antigen-1, Macrophage-1 antigen, Rats