间充质干细胞移植干预2-OA/BSA诱导的原发性胆汁性胆管炎小鼠肝内胆管上皮细胞自噬蛋白表达的变化

doi:10.3969/j.issn.1672-5069.2019.03.007

Abstract

Abstract: Objectiv To establish a reliable animal model of primary biliary cholangitis（PBC） and to investigate the therapeutic effect of umbilical cord-derived mesenchymal stem cells （UC-MSC） on STAT3 signal in intrahepatic biliary epithelial cells（IBECs） of this PBC animal. Methods C57BL/6 mice were intraperitoneally injected with 2-octynyl acid （2-OA）-bovine serum albumin （BSA） adjuvanted with Freund's adjuvant/incomplete Freund's adjuvant （CFA/IFA） or with same amount of BSA adjuvanted with IFA,or untreated as control. The model mice 22 weeks later were randomly divided into model （n=4）,MSCs transplantation （n=6） and STAT3 inhibitors-treated group（n=6）. The intrahepatic biliary tree and IBECs were obtained,and STAT3/pSTAT3,p62,LC3,PKR/pPKR,Beclin-1,eIF2α/peIF2α and LAMP-1 expression were detected by WB,and STAT3,LC3 and p62-mRNA were detected by RT-PCR. Results The lymphocyte infiltration and granuloma in portal area were found in liver tissues of model mice,while they were obviously alleviated in MSCs-or Stattic-intervened groups;the expressions of Beclin-1,STAT3 and pSTAT3 in IBECs from model intensified as compared to those in BSA-treated group,while they were weaken in MSCs-or Stattic-intervened groups;p62 mRNA levels decreased in model and MSC transplanted grous as compared to that in BSA-treated group,and STAT3 mRNA level in model decreased as compared to that in BSA-treated group. Conclusion Our findings indicate that MSC transplantation might regulate the autophagy and decrease the expression of STAT3 signals in mice with primary biliary cholangitis.

Key words: Primary biliary cholangitis, Mesenchymal stem cells, Autophagy, STAT3 signals

Zhu Yun, Yao Genhong, Tang Xiaojun. Mesenchymal stem cell transplantation alleviating hepatic injury by modulating intrahepatic biliary epithelial cell autophagic flux in mice with 2-OA-BSA-induced primary biliary cholangitis[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(3): 337-340.

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Mesenchymal stem cell transplantation alleviating hepatic injury by modulating intrahepatic biliary epithelial cell autophagic flux in mice with 2-OA-BSA-induced primary biliary cholangitis

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