Abstract: Objective This study aimed to explore the implication of ubiquitinase E2T (UBE2T) expression in cancerous tissues in patients with gallbladder cancer. Methods 50 patients with gallbladder cancer were included in this study between January 2015 and December 2017. The cancerous and adjacent noncancerous tissues as well as 50 normal gallbladder tissues were collected, and the relative expression of UBE2T was detected by Western bloting. All patients with gallbladder cancer underwent radical tumor resection and followed-up for up to 36 months. The multivariate Logistic regression analysis was applied to predict the risk factors for poor prognosis of patients with gallbladder cancer after operation. Results The relative expression ofUBE2T in cancerous tissue was (2.9±0.4), much higher than (1.5±0.3, P<0.05) in noncancerous or (1.7±0.3, P<0.05) in normal tissues; there were a significant differences as respect to the expression of UBE2T gallbladder between differentTNM stages, invasions, lymph node metastasis, remote metastasis and pathological classification (P<0.05); after 21.0 month follow-up, the Logrank analysis showed that there was a significant difference between 12.0 month survival in 31 patients with high cancerous UBE2T expression and 25.0 month (P<0.05) in 19 patients with low cancerous UBE2T expression; the multivariate Logistic analysis demonstrated that the poor TNM stage [OR(95%CI):1.9(1.5-2.4)], strong cancerous UBE2T expression [OR(95%CI):2.5(2.1-2.9)], tumor invasion [OR(95%CI):2.3(1.8-3.0)], lymph node metastasis [OR(95%CI):1.2(1.0-1.8)], remote metastasis [OR(95%CI):2.1(1.7-2.8)] and poor pathological classification [OR(95%CI): 1.6(1.3-2.2)] were the risk factors for poor prognosis of patients with gallbladder cancer after operation (P<0.05). Conclusion The expression of UBE2T protein in gallbladder tissues of patients with gallbladder cancer is significantly intensified, which might be related to the poor prognosis.

Key words: Gallbladder carcinoma, Ubiquitinase E2T, Prognosis