草质素对非酒精性脂肪性肝炎大鼠肝脂肪变和肝内氧化应激的影响

doi:10.3969/j.issn.1672-5069.2020.05.006

Abstract

Abstract: Objective The aim of this study was to investigate the effects of herbacetin on hepatic steatosis and intrahepatic oxidative stress in rats with non-alcoholic steatohepatitis (NASH). Methods The high-fat diet was applied to induce SD rats for 12 weeks to establish models of NASH, and at the same time, the herbacetin at dose of 20 mg·kg^-1.d^-1 and 40 mg·kg^-1.d^-1 were administered daily intragastricly. Serum free fatty acid (FFA) , malondialdehyde (MDA) in liver homogenate, and activities of superoxide dismutase (SOD), peroxidase (CAT), glutathione peroxidase (GSH-Px) and heme oxidase (HO-1) were detected. The hepatic protein expression of Nrf2, cytochrome P450 enzyme 2E1 (CYP2E1) and cytochrome P450 enzyme 4A (CYP4A) were assayed by Western blotting. Results The body weight, liver weight and liver indexin model group were (499.2±14.1) g, (15.9±0.6) g and (3.2±0.1)% , much higher than in the control; the body weight, liver weight and liver index in low-dose and high-dose of herbacetin-intervened group were much lower than those in the model (P<0.05); serum FFA level in the model was (521.5±52.3) mmol/L, significantly higher than in the control; the liver homogenate levels of SOD, CAT, GSH-Px and HO-1 in the model were (294.2±35.4) U/mg, (19.5±6.4) U/mg, (362.4±104.2) U/mg and (4.1±0.4) ng/mg, all significantly lower than in the control; the liver homogenate levels of SOD, GSH-Px and HO-1 in low-dose and high-dose of herbacetin-intervened group were much higher than those in the model (P<0.05); the hepatic Nrf2 expression in the model was significantly weaker than that in the control, while the hepatic CYP2E1 and CYP4A expression was much stronger than in the control (P<0.01); the hepatic Nrf2 expression in low-dose and high-dose of herbacetin-intervened group increased(P<0.01), while hepatic CYP2E1 and CYP4A expression deeply decreased as compared to those in the model (P<0.05). Conclusion The herbacetin could improve lipid accumulation and oxidative stress disorder in the liver tissues of rats with NASH, and the anti-oxidation mechanism might be related to the regulation of Nrf2 gene expression.

Key words: Non-alcoholic steatohepatitis, Herbacetin, Liver steatosis, Oxidative stress, Rats

Wang Yu, Wang Nan, Liu Yuanyuan, et al.. Effects of herbacetin onhepatic steatosis and intrahepatic oxidative stress in rats with non-alcoholic steatohepatitis[J]. Journal of Practical Hepatology, 2020, 23(5): 626-629.

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Effects of herbacetin onhepatic steatosis and intrahepatic oxidative stress in rats with non-alcoholic steatohepatitis

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