二氢姜黄素体外处理BRL-3A细胞对细胞凋亡通路的影响

doi:10.3969/j.issn.1672-5069.2020.05.005

Abstract

Abstract: Objective The aim of this study was to investigate the effects of dihyd rocurcumin (DHC) on apoptotic pathway in palmitic acid (PA)-induced BRL-3A cell apoptosis in vitro. Methods The non-alcoholic steatohepatitis (NASH) cell model was established by PA induction in BRL-3A cells. The survival rate of BRL-3A cells was detected by MTT assay. The cells were divided into control with normal medium, model (0.25 mM PA) and DHC intervention group with 16, 32, 64 μM DHC culture. The content of lactate dehydrogenase (LDH) in cell culture supernatant was detected. The apoptosis was detected by flow cytometry. The cell mitochondrial membrane potential (MMP), activity of apoptosis-related protease Caspase-3 and Caspase-9 were detected and the protein expression of mitochondrial apoptosis-related proteins Bcl-2 and Bax was detected by Western blotting. Results The supernatant LDH level, activities of Caspase-3 and Caspase-9 in model group were (100.1 ± 4.2)%, (1.0 ± 0.2)% and (1.0 ± 0.1)%, much higher than in the control, while the MMP was lower than that in control group ; the LDH, Caspase-3 and Caspase-9 in 32 μM DH-intervened cells were (84.4±2.4)%, (0.8±0.1)% and (0.7±0.1)%, significantly lower than those in the model (P<0.05), whilethe MMP was (0.6±0.1)%, much higher than that in the model (P<0.05); the apoptotic rate in the model group was significantly increased as compared to that in the control, while it decreased greatly as compared with model in DHC-intervened group; the relative expression of Bcl-2 protein in model group was lower than that in control group , while the relative expression of Bax protein was higher than that in control group , but the relative expression of Bcl-2 protein in 32 μM DHC- treated group and 64 μM DHC-treated group were (1.3 ± 0.1) and (1.9 ± 0.2), much higher than those in the model group (P<0.05), and the relative expression of Bax protein were (0.7 ± 0.1) and (0.6 ± 0.1), much lower than those in the model group (P<0.05). Conclusion DHC might relief the apoptosis caused by PA inBRL-3A cells by inhibiting the mitochondria-mediated apoptotic pathway.

Key words: BRL-3A cells, Dihydrocurcumin, Non-alcoholic steatohepatitis, Mitochondria, Apoptotic pathway

Wu Gang, Chen Xunjun, Zhang Wanli, et al.. Effects of dihyd rocurcumin on apoptotic pathway in palmitic acid-induced BRL-3A cell apoptosis in vitro[J]. Journal of Practical Hepatology, 2020, 23(5): 622-625.

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Effects of dihyd rocurcumin on apoptotic pathway in palmitic acid-induced BRL-3A cell apoptosis in vitro

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