胆石症患者血清微小RNA -122、高迁移率族蛋白1和角蛋白-18水平及其临床意义*

doi:10.3969/j.issn.1672-5069.2019.02.033

Abstract

Abstract: Objects To investigate serum microRNA-122（miR-122）,high mobility group protein 1 （HMGB1） and caspase-aspartate proteolytic keratin-18 （CCK-18） level changes in patients with cholelithiasis and their clinical significance. Methods 201 patients with cholelithiasis and 103 healthy persons admitted to our hospital between January 2015 and February 2018 were enrolled,and serum miR-122,HMGB1 and CCK-18 levels were compared between the two groups. Logistic regression analysis was applied to analyze the risk factors affecting the progression of simple cholelithiasis into complex cholelithiasis. The efficacy of serum markers in the diagnosis of complex cholelithiasis was analyzed by area under the receiver operating characteristic curve （AUC）. Results Serum levels of miR-122,HMGB1 and CCK-18 in patients with cholelithiasis were 4.7（0.9,14.3）,（2.5±0.7） ng/mL and（126.8±20.5） U/L,respectively,significantly higher than[0.9 （0.4,3.1）,（2.1±0.4） ng/mL and （101.4±18.7） U/L,all P<0.05] in control;serum miR-122,HMGB1 and CCK-18 in 97 patients with simple cholelithiasis were 3.5（0.7,8.5）,（2.2±0.5） ng/mL and （112.4±19.4） U/L,respectively,significantly lower than in 104 patients with complex cholelithiasis [5.8 （0.9,16.2）,（2.8±0.8） ng/mL and （140.2±23.5） U/L,respectively,P<0.05];univariate and multivariate Logistic regression analysis showed that liver cirrhosis,peritoneal effusion,serum miR-122,HMGB1 and CCK-18 were the risk factors related to simple cholelithiasis turned to complex one;our findings also showed that the combination of the three serum parameters increased the diagnostic efficacy of complex cholelithiasis[AUC 0.819（0.758~0.962） vs. AUC miR-122 0.724（0.657~0.815）,AUC HMGB 1 0.701（0.633~0.804） or AUC CCK-18 0.767（0.702~0.873）]. Conclusion The patients with cholelithiasis and high serum mir-122,HMGB1 and CCK-18 levels might be sophisticated in disease severity,and should be taken into consideration and carefully managed.

Key words: Cholelithiasis, Complex cholelithiasis, MicroRNA-122, High mobility group protein 1, Cysteine aspartate protease cleavage keratin-18

Cheng Tiejun, Zhang Mingzhi. Serum levels of microRNA-122,high mobility group protein 1 and caspase-cleaved keratin-18 in patients with cholelithiasis and their clinical significance[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(2): 285-288.

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Serum levels of microRNA-122,high mobility group protein 1 and caspase-cleaved keratin-18 in patients with cholelithiasis and their clinical significance

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