Abstract: Objective To study the changes and significance of serum cell migration induced hyaluronan-binding protein （CEMIP），carbohydrate antigen 19-9 （CA19-9 and CA242 in patients with pancreatic cancer （PC）. Methods A total of 92 patients with PC，105 patients with benign pancreatic diseases，and 83 healthy volunteers were recruited in our hospital beteen April 2013 and August 2016，and serum CEMIP levels were measured by ELISA，serum CA19-9 and CA242 levels were measured by radioimmunoassay. The area under the receiver-operating characteristic curve（AUC） was applied to evacuate the diagnostic efficacy，Kaplan-Meier and Cox proportional hazard model was applied for the survival analysis and Logistic regression was applied to analyze the risk factors impacting the survival. Results Serum levels of CEMIP，CA19-9 and CA242 in patients with PC were 0.7（0.4，1.0） ng/mL，180.1（89.1，230.3） U/mL and 61.7（20.7，93.5） U/mL，respectively，much higher than those in patients with benign pancreatic disease or healthy controls（P<0.05）；the AUC of combined serum CEMIP，CA19-9，and CA242 in diagnosis of patients with PC was 0.966，and its diagnostic efficacy was higher than either of the three parameters alone；serum CEMIP，CA19-9，and CA242 levels could predict effectively the survival of patients with PC at the end of 1 year after radical removal of the tumor；Kaplan-Meier and Cox multivariate analysis showed that tumor differentiation，vascular invasion， postoperative chemotherapy，serum CEMIP≥0.7 ng/mL，CA19-9≥90.3 U/mL，and CA242≥32.8 U/mL were the independent risk factors influencing the survival of patients with PC after operation. Conclusion The detection of serum CEMIP，CA19-9 and CA242 levels in patients with PC might help to diagnose the disease and evaluate the prognosis.

Key words: Pancreatic cancer, Cell migration induced hyaluronan-binding protein, Carbohydrate antigen 19-9, Carbohydrate antigen 242, Diagnosis, Survival