肝肺综合征大鼠血细胞因子和肺血管巨噬细胞观察

doi:10.3969/j.issn.1672-5069.2014.02.016

Abstract

Abstract: Objective To evaluate pulmonary intravascular macrophage accumulation and changes of plasma level of endotoxin in rats with hepatopulmonary syndrome （HPS）. Methods Thirty Wistar rats receiving sham surgery or common bile duct ligation （CBDL） were sacrificed 5 weeks after surgery；Arterial blood were obtained for arterial blood gas analysis，and plasma were obtained for measurement of endotoxin（by Limulus test），nitric oxide （by Nitrale reduetase） and tumor necrosis factor alpha （by ELISA）；The lung and liver tissues were collected for histological evaluation. Results As compared to sham group，rats in CBDL group at fifth week had lower PaO2 [（64.2±7.3） mmHg vs. （97.7±4.5） mmHg，P<0.05]， higher plasma levels of endotoxin，nitric oxide and tumor necrosis factor-ɑ [（0.2297±0.0362） Eu/ml vs. （0.0938±0.0309） Eu/ml，（56.2±9.5） μmol/L vs.（32.9±4.3） μmol/L，and（1.5±0.2） ng/ml vs.（0.4±0.1） ng/ml，respectively，P<0.05]；The histological examination revealed that fibrosis in the liver and the expansion and congestion of the pulmonary capillaries together with pulmonary intravascular macrophage accumulation in the lung，were observed in CBDL-treated rats. Conclusions In rats with experimental model of hepatic cirrhosis，the elevated level of plasma endotoxin，accumulation of pulmonary intravascular macrophages and increased secretion of inflammatory cytokines may contribute to intrapulmonary vasodilation and hyoxemia as a result of hepatopulmonary syndrome.

Key words: Hepatopulmanory syndrome, Macrophages, Endotoxin, Bile duct ligation, Rats

Guo Yuening，Wang Liguo，Wang Xiazhen，et al.. Changes of serum cytokines and pulmonary intravascular macrophages in rats with hepatopulmonary syndrome[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2014, 17(2): 176-179.

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Changes of serum cytokines and pulmonary intravascular macrophages in rats with hepatopulmonary syndrome

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