Abstract: Objective To study the changes of serum hepatitis B virus e antigen and HBV DNA load in patients with hepatitis B treated with lamivudine. Methods Emzyme chemiluminescence immunoassay and real-time fluorescent quantitative PCR were performed in detecting the level of serum HBeAg and HBV DNA in 64 patients in 12-month lamivudine therapy. Results HBV DNA turned negative in 49（76.6%）out of the 64 patients,and HBeAg reduced to the level undetectable in 17（26.6%）;11 patients were nonreponded to lamivudine treatment with their serum HbeAg and HBV DNA positive;Four patients had their HBV DNA transiently negative, but it turned to be positive again after 6 month. Conciusion Detecting serum HBeAg dynamically might be useful in evaluation of lamivadine therapeutic effect.

Key words: Hepatitis B, HBeAg, Enzyme chemiuminescence immunoassay