IL-22和SOCS3在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎发病中的作用探讨*

doi:10.3969/j.issn.1672-5069.2014.04.017

实用肝脏病杂志 ›› 2014, Vol. 17 ›› Issue (4): 400-404.doi: 10.3969/j.issn.1672-5069.2014.04.017

IL-22和SOCS3在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎发病中的作用探讨^*

李咏, 陆玉蕾, 吴婷, 韩梅芳, 宁琴

430030 武汉市华中科技大学同济医学院附属同济医院感染性疾病研究所/感染病科（李咏,吴婷,韩梅芳,宁琴）; 广西医科大学附属肿瘤医院（陆玉蕾）

收稿日期:2014-02-07 出版日期:2014-08-30 发布日期:2016-04-11
通讯作者: 宁琴,E-mail:qning@vip.sina.com
作者简介:李咏,男,32岁,博士研究生。主要从事病毒性肝炎发病机制研究。E-mail:vamos_yong@hotmail.com
基金资助:
国家自然科学基金项目（编号：NSFC81171558, NSFC81271808,NSFC81030007）; 教育部创新团队项目（编号：IRT1131）

Effect of IL-22 and SOCS3 in MHV-3 induced chronic viral hepatitis in mice

Li Yong, Lu Yulei, Wu Ting

Department and Institute of Infectious Disease,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 400030,Hubei Province,China

Received:2014-02-07 Online:2014-08-30 Published:2016-04-11

摘要/Abstract

摘要： 目的探讨鼠3型肝炎病毒（MHV-3）诱导的慢性病毒性肝炎C3H/Hej小鼠肝组织白细胞介素-22（IL-22）和细胞因子信号抑制因子3（SOCS3）mRNA水平的变化。方法给C3H/Hej小鼠腹腔注射MHV-3（100 pfu）,诱导慢性病毒性肝炎模型,采用实时荧光定量PCR 法检测MHV-3感染后0、5、10、15和20 d时小鼠肝组织IL-22和SOCS3 mRNA水平的变化。结果模型鼠肝组织IL-22和SOC3 mRNA水平在0 d时分别为（0.26±0.15）%和（6.35±2.21）%,在感染后10、15和20 d后IL-22水平分别为（6.28±2.79）%、（2.50±1.24）%和（2.73±0.85）%,SOC3水平分别为（16.92±4.39）%、（14.06±4.09）%和（13.36±1.89）%,均较0 d时显著增高（P<0.05）,且在第10 d达到最高峰。结论IL-22和SOCS3可能参与了MHV-3诱导的小鼠慢性病毒性肝炎的发生发展过程。

关键词: 鼠3型肝炎病毒, 慢性病毒性肝炎, 白细胞介素-22, 细胞因子信号抑制因子3, 小鼠

Abstract: Objective To investigate the changes of interleukin-22 （IL-22） and suppressor of cytokine signaling 3（SOCS3） mRNA in murine hepatitis virus 3（MHV-3） induced chronic viral hepatitis in C3H/Hej mice. Methods Chronic viral hepatitis in C3H/Hej mice was induced by intra-peritoneal injection of MHV-3 virus at dose of 100pfu. At day 0,5,10,15 and 20 post-infection,IL-22 and SOCS3 mRNA in the liver tissues were detected by real-time PCR. Results IL-22 and SOCS3 mRNA level in livers of model mice at day 0 were （0.26±0.15）% and（6.35±2.21）%,respectively. At day 10,15 and 20,IL-22 mRNA levels in the liver tissues were （6.28±2.79）%,（2.50±1.24）% and（2.73±0.85）%,respectively,and SOCS3 mRNA levels were（16.92±4.39）%,（14.06±4.09）% and （13.36±1.89）%,respectively, all of which were markedly increased than baseline（P<0.05）. The mRNA levels of IL-22 and SOCS3 both peaked at day 10 post MHV-3 infection. Conclusions IL-22 and SOCS3 may play an important role in the pathogenesis of MHV-3-induced chronic viral hepatitis in mice.

Key words: Murine hepatitis virus 3, Chronic viral hepatitis, Interleikin-22, Suppressor of cytokine signaling 3, Mouse

李咏, 陆玉蕾, 吴婷, 韩梅芳, 宁琴. IL-22和SOCS3在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎发病中的作用探讨^*[J]. 实用肝脏病杂志, 2014, 17(4): 400-404.

Li Yong, Lu Yulei, Wu Ting. Effect of IL-22 and SOCS3 in MHV-3 induced chronic viral hepatitis in mice[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2014, 17(4): 400-404.

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IL-22和SOCS3在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎发病中的作用探讨^*

Effect of IL-22 and SOCS3 in MHV-3 induced chronic viral hepatitis in mice

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