关键词: 酒精性肝病, 脂肪酶, 过氧化脂质, 一氧化氮合成酶, 肠道菌群

Abstract: Objective The aim of this study was to analyze the changes of serum lipase (LP), lipid peroxide (LPO), nitric oxide synthase (NOS) and intestinal flora distribution in patients with alcoholic liver disease (ALD). Methods 88 patients with ALD 【including alcoholic fatty liver (AFL) in 36, alcoholic hepatitis (AH) in 27 and alcoholic liver cirrhosis (ALC) in 25 】 and 40 healthy persons were recruited in our hospital between January 2017 and June 2019, were serum LP, LPO and NOS levels were detected. The intestinal bacteria species and the numbers of major intestinal bacteria were separated and characterized. Results Serum LP level in patients with ALC was (6.2±1.9) μ g/L, significantly higher than [(3.6±1.1) μ g/L, P<0.05]in patients with AH, or [(1.6±0.4) μ g/L, P<0.05]in patients with AFL, or [(1.1±0.2) μ g/L, P<0.05]in healthy control group; serum LPO level in ALC group was (7.2±2.1) μ mol/l, much higher than [(4.7±1.6) μ mol/l, P<0.05]in AH group, or [(3.3±1.0) μ mol/l, P<0.05]in AFL group, or [(2.1±0.5) μ mol/l,P<0.05]in healthy control; serum NOS level in ALC group was (7.8 ±2.6) U/ml, significantly higher than [(4.9±1.7) U/ml, P<0.05]in AH group, or [(3.5±1.2) U/ml, P<0.05]in AFL group, or [(1.3±0.4) U/ml, P<0.05]in healthy control; the fecal bacteria strains in ALC group was (20.7±1.1), much lower than [(30.9 ±1.8), P<0.05]in AFL group, or [(31.7±2.5), P<0.05]in healthy control, while the bacteria strains in AH group was (21.4±1.2), significantly lower than in AFL or in healthy control group (P<0.05); the number of Escherichia coli in ALC group was (9.2±1.8) IgCFU/g, significantly higher than [(8.3±1.4) IgCFU/g, P<0.05]in AH group, or [(6.8±1.5) IgCFU/g, P<0.05]in AFL group, or [(7.1±1 1.3) IgCFU/g, P<0.05]in healthy control group; the number of Enterococcus faecalis in ALC group was (9.5 2.3) IgCFU/g, significantly higher than [(7.1±1.3) IgCFU/g, P<0.05]in patients with AFL, or [(6.5±1.5) IgCFU/g, P<0.05]in healthy control group, or[ (8.6±1.5) IgCFU/g, P<0.05]in AH group; the number of Bifidobalcterium in ALC group was (6.3 0.7) IgCFU/g, much lower than [(7.2±1.1) IgCFU/g, P<0.05]in AH group, or [(7.9±1.4) IgCFU/g, P<0.05]in patients with AFL, or [(8.5±1.4) IgCFU/g, P<0.05]in healthy control. Conclusion Patients with ALD have increased serum LP, LPO and NOS levels and intestinal microecology disorders. As the disease progresses or turns to be AH or ALC, the changes become obvious.

Key words: Alcoholic liver disease, Lipase, Lipid peroxide, Nitric oxide synthase, Intestinal flora