关键词: 酒精性肝病, 细胞因子, 肠道菌群

Abstract: Objective The purpose of this study was to investigate the changes of serum cytokines and intestinal flora distribution in patients with alcoholic liver disease（ALD）.Methods 34 patients with ALD and 34 healthy individuals were recruited in our hospital,and serum tumor necrosis factor-α（TNF-α）,interleukin-6 and NF-κB levels were detected by ELISA. The intestinal flora distribution were separated and characterized.Results Fasting blood glucose,total cholesterol,TG,LDL-C and HDL-C levels in patients with ALD were（5.3±1.8） mmol/L,（2.3±0.9） mmol/L,（5.1±1.8） mmol/L,（2.1±0.6） mmol/L and（0.9±0.2） mmol/L,not significantly different as compared to（5.2±1.3） mmol/L,（2.2±1.1）mmol/L,（5.1±1.6） mmol/L,（2.0±1.5） mmol/L and （0.8±0.1） mmol/L in the healthy persons（P>0.05）;serum ALT,AST,GGT and bilirubin levels were（99.5±41.8） U/L,（106.9±55.7） U/L,（229.4±148.3） U/L and（35.3±8.6） μmol/L,significantly higher than【（35.6±9.7） U/L,（36.2±8.4） U/L,（46.7±14.5） U/L and（16.4±2.3） μmol/L,respectively,P<0.05】 in the control;serum TNF-α,IL-6 and N F-κB levels were （9.8±3.4） ng/L,（0.9±0.4） pg/mL and（3.7±6.3） ng/L,much higher than 【（5.7±2.1） ng/L,（0.5±0.3） pg/mL and （0.5±0.4） ng/L,P<0.05】 in the control;the enterococcus faecalis and escherichia Coli counts in patients with ALD were （8.7±1.4） lg CFU/g/g and （7.6±1.2） lg CFU/g/g significantly higher than [（7.2±1.3） lg CFU/g/g and （6.6±1.5） lg CFU/g/g,respectively,P<0.05] in the control,while the bifidobacterium counts were（7.1±1.2） lg CFU/g/g,much lower than [（8.9±1.3） lg CFU/g/g,P<0.05] in the control. Conclusion The intestinal flora disorders and high levels of serum cytokines exist in patients with ALD,which might take part in the pathogenesis of the disease.

Key words: Alcoholic liver disease, Cytokines, Intestinal flora