肝源性糖尿病患者肝组织干扰素诱导蛋白-10表达变化*

doi:10.3969/j.issn.1672-5069.2019.05.019

摘要/Abstract

摘要： 目的观察肝源性糖尿病患者肝组织和血清干扰素诱导蛋白-10（IP-10）水平变化,并分析其在肝源性糖尿病发病过程中的可能作用。方法 2016年3月~2018年5月我院诊治的乙型肝炎肝硬化并发肝源性糖尿病患者70例和不伴有糖尿病的乙型肝炎肝硬化患者70例,采用ELISA法检测血清IP-10水平,采用PCR法和免疫组化法分别检测肝组织IP-10 mRNA水平和蛋白表达。结果伴有糖尿病的肝硬化血清IP-10水平为（236.59±28.30） pg/mL,显著高于不伴有糖尿病的肝硬化患者【（205.31±26.92） pg/mL,P<0.001】;糖尿病患者肝组织IP-10 mRNA水平为（0.59±0.91） lg copies/ml,显著高于肝硬化患者【（0.21±0.66） lg copies/ml,P<0.05】,差异具有统计学意义;糖尿病患者肝组织IP-10表达阳性率为31.4%,显著高于肝硬化患者的12.9%,差异具有统计学意义（x2=7.002,P<0.05）。结论乙型肝炎肝硬化并发肝源性糖尿病患者血清和肝组织IP-10水平显著升高,其意义还有待于进一步探讨。

关键词: 肝硬化, 乙型肝炎, 肝源性糖尿病, 干扰素诱导蛋白-10, 肝脏病理学

Abstract: Objective To investigate hepatic expression of interferon inducible protein -10 （IP-10） in patients with hepatitis B liver cirrhosis and hepatogenic diabetes mellitus. Methods The specimens of liver tissues from 70 patients with hepatitis B-induced liver cirrhosis and hepatogenic diabetes mellitus and another 70 patients with hepatitis B-induced liver cirrhosis were obtained by liver biopsies between March 2016 and May 2018,and serum IP-10 were assayed by ELISA,and hepatic IP-10 mRNA and its protein expression were detected by PCR and immunohistochemistry. Results Serum IP-10 level in patients with hepatitis B-induced liver cirrhosis and hepatogenic diabetes mellitus was （236.59±28.30） pg/mL,significantly higher than [（205.31±26.92） pg/mL,P<0.001] in patient with liver cirrhosis without hepatogenic diabetes mellitus; hepatic IP-10 mRNA was （0.59±0.91）,significantly higher than [（0.21±0.66）,P<0.05] in patients without diabetes mellitus;the immunohistochemical staining results showed that the hepatic IP-10 expression positive rate was 31.4%,significantly higher than（12.9%,x2=7.002,P<0.05） in patients without diabetes mellitus. Conclusion The expression of IP-10 in liver tissues and serum IP-10 levels in patients with hepatogenic diabetes mellitus with underlying hepatitis B liver cirrhosis is sig-nificantly increased,which might be involved in the pathogenesis of hepatogenic diabetes mellitus through inflammatory mechanism.

Key words: Liver cirrhosis, Hepatitis B, Hepatogenic diabetes mellitus, Interferon inducible protein-10, Liver histopathology

张梅, 石秀英, 李林娟, 薛亚妮. 肝源性糖尿病患者肝组织干扰素诱导蛋白-10表达变化*[J]. 实用肝脏病杂志, 2019, 22(5): 688-691.

Zhang Mei, Shi Xiuying, Li Linjuan, et al. Hepatic expression of interferon inducible protein -10 in patients with hepatitis B liver cirrhosis and hepatogenic diabetes mellitus[J]. Journal of Practical Hepatology, 2019, 22(5): 688-691.

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