IGF-1在酒精性肝病伴发肠粘膜损伤中的作用探讨*

doi:10.3969/j.issn.1672-5069.2012.04.012

实用肝脏病杂志 ›› 2012, Vol. 15 ›› Issue (4): 305-308.doi: 10.3969/j.issn.1672-5069.2012.04.012

IGF-1在酒精性肝病伴发肠粘膜损伤中的作用探讨^*

聂, 娇, 李, 鑫, 崔培林, 徐有青

100050 北京市首都医科大学附属北京天坛医院消化内科

收稿日期:2011-12-26 出版日期:2012-08-10 发布日期:2017-03-15
通讯作者: 崔培林,E-mail:cuipl@yahoo.cn
作者简介:第一作者：聂娇女,25岁,硕士研究生。主要研究方向为酒精性肝病的防治研究。E-mail:jiao627@126.com
基金资助:
国家自然科学基金（编号：No; 30901745 ）

The effect of IGF-1 on intestinal mucous injury in mice with alcohol-induced liver disease

Nie Jiao, Li Xin, Cui Peilin, et al.

Department of Gastroenterology,Tiantan Hospital,Capital Medical University,Beijing 100050,China

Received:2011-12-26 Online:2012-08-10 Published:2017-03-15

摘要/Abstract

摘要： 目的探讨IGF-1在酒精性肝病伴发肠粘膜损伤中的作用,并观察谷氨酰胺对肠粘膜IGF-1表达的影响。方法将30只小鼠随机分为对照组、模型组和治疗组,每组10只。对照组饲喂Lieber-Decarli无酒精液体饲料,模型组和治疗组饲喂含4%乙醇Lieber-Decarli液体饲料建立酒精性肝病模型,治疗组同时给予谷氨酰胺灌胃。12周后处死小鼠,心脏采血检测血清内毒素;取肠组织进行肠形态学检查,采用免疫组织化学法检测IGF-1表达。结果模型组血清内毒素较对照组明显升高（0.38±0.05 Eu/L 对 0.13±0.02 Eu/L,P<0.05）,治疗组较模型组血清内毒素水平下降;模型组结肠损伤病理学评分较对照组明显升高（ 10.3±1.3分对4.8±1.2分,P<0.05）,治疗组结肠损伤病理学评分较模型组降低;模型组肠粘膜IGF-1 表达明显高于对照组（2.3±0.2分对 0.9±0.2分）,治疗组表达也相应的减少。结论酒精性肝病时伴有肠粘膜损伤。IGF-1在酒精性肝病伴发肠粘膜损伤中起到了修复作用。谷氨酰胺可保护肠粘膜,而且可能与IGF-1的表达有关。

关键词: 酒精性肝病, 肠粘膜损伤, 胰岛素样生长因子-1, 小鼠

Abstract: Objective To study the effect of IGF-1 on intestinal mucous injury in mice with alcohol-induced liver disease and to observe the impact of glutamine on expression of IGF-1. Methods Thirty c57 mice were randomly divided into control,model and intervention group（10 in each）. The control group were fed with Lieber Decarli liquid without ethanol,and mice in the model and intervention group were fed with 4% ethanol Lieber Decarli liquid. At the same time,glutamine was given to the mice in the intervention group. The serum endotoxin was examined after 12 weeks,and intestinal tissues were assessed with HE staining and the expression of IGF-1 in intestine was detected by immunohistochemical method. Results The serum endotoxin in the model group was much higher than that in control group（0.38±0.05 Eu/L vs. 0.13±0.02 Eu/L,P<0.05）,while it decreased in intervention group as compared with the model group;the histopathological score of colon injury in the model group was much higher than that in control group（10.3±1.3 vs 4.8±1.2,P<0.05）;the expression of IGF in the model group was much higher than that in control group（2.3±0.2 vs. 0.9±0.2,P<0.05）. Conclusion Alcoholic liver disease is always complicated with intestinal mucous injury. IGF-1 might play a role in alcoholic liver disease. Glutamine can protect the intestinal mucosa,and it may be associated with the expression of IGF-1.

Key words: Alcohol liver disease, Intestinal mucous injury, IGF-1, Mice

聂, 娇, 李, 鑫, 崔培林, 徐有青. IGF-1在酒精性肝病伴发肠粘膜损伤中的作用探讨^*[J]. 实用肝脏病杂志, 2012, 15(4): 305-308.

Nie Jiao, Li Xin, Cui Peilin, et al.. The effect of IGF-1 on intestinal mucous injury in mice with alcohol-induced liver disease[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2012, 15(4): 305-308.

参考文献

[1] Purohit V,Bode JC,Bode C,et al. Alcohol,intestinal bacterial growth,intestinal permeability to endotoxin and medical consequences:summary of a symposium. Alcohol,2008,42（5）:349-361.
[2] Baregamian N,Song J,Jeschke MG,et al. IGF-1 protects intestinal epithelial cells from oxidative stressinduced apoptosis. J Surg Res,2006,136（1）:31-37.
[3] Arak1Y,AndohA,TakizawaJ,et al. Clostridium butyricum,a probiotic derivative suppresses dextran sulfate sodium-induced experimental colitis in rats. Int J Mol Med,2004,13（4）:577-580.
[4] Gulpinar MA,Ozbeyli D,Arbak S,et al. Acute stress ameliorates colitis via central corticotropin-releasing factor and serotonin（5-HT）-3 receptors. Turk J Med Sci,2005,22（35）:13-19.
[5] Williams KI,Fuller CR,Fagin J,et al. Mesenchymal IGF-1 overexpression: paraerine effects in the intestine distinct from endocrine actions. Am J Physiol Gastm intest Liver Physiol,2002,283（4）:G875-G885.
[6] Zimmermann EM,Li L,Hou YT,et al. IGF-I induces collagen and IGFBP-5 mRNA in rat intestinal smooth muscle. Am J Physiol,1997,273（4 Pt 1）:G875-G882.
[7] Kuemmerle JF,Zhou H,Bowers JG. IGF- I stimulates human intestinal smooth muscle cell growth by regulation of G1 phase cell cycle proteins. Am J Physiol Gastrointest Liver Physiol,2004,286 （3）:G412-G419.
[8] Beale RJ,Sheery T,Lei K,et al. Early enteral supplementation with key pharmaconutrients improves sequential organ failure assessment score in critically ill patients with sepsis:Outcome of a randomized,controlled double-trial. Crit Care Med,2008,36 （1）:131-144.
[9] 崔巍,闻颖,董亚珞,等. 谷氨酰胺对体外培养肠上皮细胞屏障通透性的影响. 世界华人消化杂志,2008,16（33）:3729-3733.
[10] Mathurin P,Deng QG,Keshavarzian A,et al. Exacerbation of alcoholic liver injury by enteral endotoxin in rats. Hepatology, 2000,32（5）:1008-1017.
[11] Robinson GM,Orrego H,Israel Y,et al. Low-molecular-weight polyethylene glycol as a probe of gastrointestinal permeability after alcohol ingestion. Dig Dis Sci,1981,26（11）:971-977.
[12] Tamai H,Kato S,Horie Y,et al. Effect of acute ethanol administration on the intestinal absorption of endotoxin in rats. Alcohol Clin Exp Res,2000,24（3）:390-394.
[13] Napolitano LM,Koruda MJ,Zimmerman K,et al. Chronic ethanol intake and burn injury:evidence for synergistic alteration in gut and immune integrity. J Trauma,1995,38（2）:198-207.
[14] Atkinson KJ,Rao RK. Role of protein tyrosine phosphorylation in acetalde-induced disruption of epithelial tight junctions. Am J Physiol,2001,280:G1280-G1288.

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IGF-1在酒精性肝病伴发肠粘膜损伤中的作用探讨^*

The effect of IGF-1 on intestinal mucous injury in mice with alcohol-induced liver disease

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