关键词: 胆石症, 急性胰腺炎, 腹腔镜胆囊切除术, 氧化应激反应

Abstract: Objective To investigate the inflammatory and oxidative stress in patients with acute pancreatitis （AP） and underlying cholelithiasis after laparoscopic cholecystectomy （LC）. Methods A total of 150 patients with cholelithiasis complicated by AP were recruited in our hospital,and 84 cases were treated with LC and 66 cases received laparotomy after the AP was controlled. Serum interleukin-6（IL-6）,IL-10,C-reactive protein （CRP）,malondialdehyde （MDA）,oxidized low density lipoprotein （ox-LDL） and paranoxonase （PON-1） were detected by ELISA,and serum 8-dihydro-8-oxoguanine（8-OHG） was detected by high-performance liquid chromatography. Results Five days after operation,serum IL-6 level in LC group was（42.3±4.8） μg/L,much lower than【（57.7±5.1）μg/L,P<0.05】 in patients receiving laparotomy,serum IL-10 level was（64.3±5.3） pg/ml,much higher than【（51.2±4.2）pg/ml,P<0.05】 in laparotomy group,serum CRP level was（15.8±5.7） μg/L,much lower than【（38.4±6.8）μg/L,P<0.05】 in laparotomy group;serum MDA level was（3.3±0.9） nmol/mL,much lower than【（5.4±1.8）nmol/mL,P<0.05】 in laparotomy group,serum ox-LDL level was（63.2±11.7） ng/ml,much lower than 【（72.3±11.0）ng/ml,P<0.05】 in laparotomy group,serum PON-1 level was（116.3±10.2） U/ml,much higher than 【（104.5±11.4）U/ml,P<0.05】 in laparotomy group,and serum 8-OHG level was（0.5±0.2） ng/ml,without significant difference as compared to（0.6±0.2）ng/ml（P>0.05） in laparotomy group. Conclusion The LC is practical in clinical practice in dealing with patients with AP and underlying cholelithiasis after AP is controlled,which might inhibit stress response in this settings.

Key words: Cholelithiasis, Acute pancreatitis, Laparoscopic cholecystectomy, Oxidative stress reaction