酒精性肝病患者肝组织cyp2e-3基因水平变化

doi:10.3969/j.issn.1672-5069.2016.05.013

实用肝脏病杂志 ›› 2016, Vol. 19 ›› Issue (5): 561-564.doi: 10.3969/j.issn.1672-5069.2016.05.013

酒精性肝病患者肝组织cyp2e-3基因水平变化

王转国, 付强, 乐永红, 庄小芳, 王宏峰

830000乌鲁木齐新疆维吾尔自治区中医院肝病科

收稿日期:2016-01-02 出版日期:2016-09-10 发布日期:2016-10-12
通讯作者: 王宏峰,E-mail1xjwlmqwhf@sina.cn
作者简介:王转国,男,32岁,硕士研究生,主治医师。 E-mail：254528425@qq.com

Hepatic cyp2e-3 gene expression in patients with alcohol liver disease

Wang Zhuanguo, Fu Qiang, Le Yonghong, et al.

Department of Liver Diseases,Chinese Medicine Hospital,Urumqi 830000,Xinjiang Uygur Autonomous Region,China

Received:2016-01-02 Online:2016-09-10 Published:2016-10-12
Contact: Corresponding author:Guan Yujuan,E-mail:gz8hgyj@126.com

摘要/Abstract

摘要： 目的探讨细胞色素cyp2e-3基因在酒精性肝病（ALD）发病中的作用。方法选择2013年2月至2015年2月我院收治的ALD患者126例,按照病程分为A组40例（患病时间在1年以内）、B组43例（患病时间在1~3年）和C组43例（患病时间在3年以上）,选择同期来我院健康体检的健康人121例作为对照。常规行肝活检,采用荧光定量PCR技术检测肝组织cyp2e-3基因水平,采用酶联免疫吸附法检测血清cyp2e-3蛋白水平,采用Western blotting法和免疫组织化学染色法检测肝组织cyp2e-3蛋白表达情况。结果ALD患者肝组织cyp2e-3基因水平（34.8±1.7）显著高于对照组【（6.6±0.7,P<0.05】;经过ELISA法检测,发现与正常人相比,不同组ALD患者血清cyp2e-3水平显著升高;经Western-blotting法检测,发现与正常人比,不同组ALD患者肝组织cyp2e-3水平显著升高;随着患病时间的延长,肝组织cyp2e-3基因表达量逐渐升高,cyp2e-3蛋白主要集中在肝细胞膜处表达,主要特征为大小不均匀的棕黄色小颗粒。结论酒精性肝病患者肝组织和血清cyp2e-3水平显著升高,因此推断cyp2e-3基因的激活可能在一定程度上促进了酒精性肝病的发生。

关键词: 酒精性肝病, cyp2e-3基因, 基因表达

Abstract: To explore the relationship between cyp2e-3 gene expression and alcoholic liver disease. Methods In the present study,we recruited 126 patients with alcohol liver disease（ALD） in our hospital from February 2013 to February 2015,and the patients were divided into group A（n=40,with the disease history less than one year）,group B（n=43,with the disease history one to three years）,and group C（n=43,with the disease history greater than three years）. We selected 121 healthy subjects at the same period for control. The hepatic cyp2e-3 mRNA was detected by quantitative PCR,serum cyp2e-3 levels were assayed by ELISA,and hepatic cyp2e-3 expression were detected by Western-blotting or stained immunohistochemistry. Results The hepatic cyp2e-3 mRNA levels in patients with ALD were（34.8±1.7）,much higher than【（6.6±0.7,P<0.05】 in healthy persons;serum cyp2e-3 levels in patients with ALD were higher than in control too;Western-blotting and SP detection found that cyp2e-3 expression in liver tissues in group A,group B,and group C were much stronger than in healthy persons as no cyp2e-3 expression were found in the latter hepatic tissues. Conclusion The activated expression of cyp2e-3 gene in liver tissues in patients with ALD might play a role in the occurrence of liver disease,and it warrants further study.

Key words: Alcoholic liver disease, cyp2e-3 gene, Expression

王转国, 付强, 乐永红, 庄小芳, 王宏峰. 酒精性肝病患者肝组织cyp2e-3基因水平变化[J]. 实用肝脏病杂志, 2016, 19(5): 561-564.

Wang Zhuanguo, Fu Qiang, Le Yonghong, et al.. Hepatic cyp2e-3 gene expression in patients with alcohol liver disease[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(5): 561-564.

参考文献

[1] 王志宁. 酒精性肝病的临床治疗分析.中国民族民间医药,2012,17（4）:294-295.
[2] Boccia S,De Lauretis A,Gianfagna F,et al. CYP2E1PstI/RsaI polymorphism and interaction with tobacco,alcohol and GSTs in gastric cancer susceptibility:a meta-analysis of the literature. Carcinogenesis,2007,28（1）:101-106.
[3] Gonzalez A,Ramirez V,Cuenca P,et al. Polymorphisms in detoxification genes CYP1A1,CYP2E1,GSTT1 and GSTM1 in gastric cancer susceptibility. Revista de Biología Tropical,2004,52（3）:591-600.
[4] 江韶辉.酒精性肝病的临床治疗分析.医学信息（中旬刊）, 2011,13（2）:145-147.
[5] 陈军. 中药降酶汤加西医常规护肝治疗慢性脂肪肝、酒精性肝病临床疗效观察. 亚太传统医药,2015,11（8）:75-76.
[6] Kang JW,Wilkerson HW,Doty SL. Mammalian cytochrome CYP2E1 triggered differential gene regulation in response to trichloroethylene（TCE） in a transgenic poplar. Funct Integrat Genomics,2010,10（3）:417-424.
[7] 梁铭. 酒精性肝病诊治与预防的临床研究分析. 当代医学.2011,17（27）:55-56.
[8] Song BJ,Cederbaum AI. Ethanol-inducible cytochrome P450 （CYP2E1）:Biochemistry,molecular biology and clinical relevance:1996 Update.Alcohol Clin Experiment Res,1996,20（8）:138A-146A.
[9] 叶新平,彭涛,黎乐群. ALDH2和CYP2E1基因多态性及饮酒习惯与肝细胞癌易感性的关系. 卫生研究,2010,39（1）:42-45.
[10] Bose S,Tripathi DM,Shiv K. Genetic polymorphisms of CYP2E1 and DNA repair genes HOGG1 and XRCC1:Association with hepatitis B related advanced liver disease and cancer.Gene,2013,19（2）:231-237.
[11] Naselli F,Catanzaro I,Caradonna F. Role and importance of polymorphisms with respect to DNA methylation for the expression of CYP2E1 enzyme. Gene,2013,536（1）:29-39.
[12] Kato S,Tajiri T,Matsukura N,et al. Genetic polymorphisms of aldehyde dehydrogenase 2,cytochrome p450 2E1 for liver cancer risk in HCV antibody-positive Japanese patients and the variations of CYP2E1 mRNA expression levels in the liver due to its polymorphism. Scan J Gastroenterol,2003,38（8）:886-893.
[13] Trafalis DT,Karamanakos PN. CYP2E1 and risk of chemically mediated cancers. Exp Opin Drug Metab Toxicol,2010,6（3）:307-319.
[14] Kenneth C,Villanueva P,Manolis K. Polymorphisms in GSTT1,GSTZ1,and CYP2E1,disinfection by-products,and risk of bladder cancer in Spain. Environment Health Perspect,2010,118（11）:1545-1550.
[15] 燕速,白振忠,王海洁. CYP2E1 DraⅠ基因遗传多态性与青海省胃癌人群易感性的相关性研究. 中国癌症杂志,2013,35（4）:273-278.
[16] 谢瑞莲,管晓翔,刘全俊. 细胞色素CYP450 2E1基因Ras Ⅰ/Pst Ⅰ位点多态性与肺癌易感性的系统评价.南京医科大学学报（自然科学版）,2007,27（9）:911-915.
[17] 迪吉,赵君慧. 青海地区藏族人群CYP2E1基因多态性与肝癌易感性.青海医学院学报,2013,34（2）:97-101.
[18] 杨肆敏. 71例酒精性肝病的临床诊疗分析.中国现代药物应用,2014,8（22）:122-123.
[19] Reddy VD,Padmavathi S,Gopi M,et al. Protective effect of emblica officinalis against alcohol-induced hepatic injury by ameliorating oxidative stress in rats. Indian J Clin Biochemist, 2010,25（4）:419-424.

酒精性肝病患者肝组织cyp2e-3基因水平变化

Hepatic cyp2e-3 gene expression in patients with alcohol liver disease

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