草苁蓉乙醇提取物对NAFLD大鼠肝脏炎性因子表达的影响

doi:10.3969/j.issn.1672-5069.2014.05.018

摘要/Abstract

摘要： 目的探讨草苁蓉乙醇提取物（BREE)对非酒精性脂肪性肝病（NAFLD)大鼠肝组织羟脯氨酸和炎性因子的影响。方法采用高脂饲料喂养构建大鼠NAFLD模型,大鼠被随机分为正常对照组、NAFLD模型组和BREE治疗组（500 mg·kg^-1·d^-1）。在用药8 w末,处死动物,留取血清,采用ELSIA法检测肝组织白细胞介素（IL）-1β、肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6水平;采用HE染色和免疫组化法分别观察大鼠肝脏病理学变化和检测肝组织NF-kB/p65表达。结果BREE治疗组大鼠血清ALT [（39.47±20.26） U/L]和AST[（46.48±18.52） U/L]水平较模型组显著下降[（79.32±19.05）U/L和（88.35±17.46）U/L,P<0.01],而血清还原性谷胱甘肽[（349.43±45.52 ）mg/L]水平较模型组明显升高[（265.38±28.57） mg/L,P<0.01];BREE治疗组大鼠肝组织NF-kB/p65阳性表达[（15.49±4.78）%]和羟脯氨酸含量[（14.28±3.08) mg/g]均较模型组显著下降[分别为（87.54±6.59）%和（35.47±4.53）mg/g,P<0.01],同样,IL-1β、TNF-α和IL- 6水平[分别为（34.51±5.61） pg/mL、（45.37±7.03）pg/mL）和（18.98±5.04）pg/mL]均较模型组显著降低[分别为（78.25±6.51） pg/mL、（85.64±6.25） pg/mL、（29.19±4.82）pg/mL,P<0.01];BREE处理组动物肝组织病理学损害程度明显减轻。结论BREE能明显改善NAFLD大鼠肝功能和肝组织胶原沉积,其机制可能与通过下调NF-κB/p65和炎症细胞因子表达有关。

关键词: 非酒精性脂肪性肝病, 草苁蓉乙醇提取物, 核转录因子κ, B/p65, 炎性细胞因子

Abstract: Objective To investigate the effect of boschniakia rossica ethanol extract（BREE） on hydroxyproline（Hyp） and inflammatory cytokines in rats with non-alcoholic fatty liver diseases （NAFLD）. Methods The model of NAFLD in rats was established by hight-fat diet administration. Rats were randomly divided into control,model and BREE treatment group （500 mg·kg^-1·d^-1）. All rats were sacrificed to harvest serum and liver tissues at the end of eighth week. Serum levels of AST,ALT and reduced glutathione （GSH） were measured,and the levels of interleukin （IL）-1β,tumor necrosis factor（TNF）-α and IL-6 in liver tissues were detected by ELSIA. HE staining and immunohistochemistry were performed for evaluation of liver pathological changes and the expression of NF-kB/p65 in liver tissues,respectively. Results Serum levels of ALT [（39.47±20.26） U/L] and AST[（46.48 ± 18.52） U/L] in rats subjected to BREE treatment significantly decreased as compared to in the model group [（79.32±19.05）U/L and （88.35±17.46） U/L,respectively,P<0.01];Serum GSH levels in BREE-treated rats [（349.43±45.52） mg/L] significantly increased than in the model [（265.38±28.57） mg/L,P<0.01];The positive rates of NF-kB/p65 [（15.49±4.78）%] and Hyp contents [（14.28±3.08） mg/g] in liver tissues in BREE-treated rats significantly decreased than in the model [（87.54±6.59）% and （35.47±4.53）mg/g,respectively,P<0.01];the IL-1β,TNF-α and IL-6 levels[（34.51±5.61） pg/mL,（45.37±7.03） pg/mL,（18.98±5.04） pg/mL,respectively] in liver tissues in BREE-treated rats were significantly lower than in the model [（78.25±6.51） pg/mL,（85.64±6.25） pg/mL,（29.19±4.82） pg/mL,respectively,P<0.01];BREE also significantly alleviated pathological changes in the liver. Conclusions BREE significantly improves liver function and collagen deposition in rats with NAFLD by down regulation of NF-κB/p65 and inflammatory cytokines in the liver.

Key words: Non-alcoholic fatty liver disease, Boschniakia rossica ethanol extract, Nuclear transcription factor κ, B/p65, Inflammatory cytokines

何松美, 刘霞, 吴煜良, 刘旭明, 殷思纯, 龙尧. 草苁蓉乙醇提取物对NAFLD大鼠肝脏炎性因子表达的影响[J]. 实用肝脏病杂志, 2014, 17(5): 519-523.

He Songmei, Liu Xia, Wu Yuliang. Impact of boschniakia rossica ethanol extract on hepatic expression of inflammatory cytokines in rats with NAFLD[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2014, 17(5): 519-523.

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