关键词: 酒精性脂肪肝, 肠源性内毒素血症, 肠黏膜通透性, 大鼠

Abstract: Objective To establish an experimental model of alcoholic fatty liver accompanied with intestinal endotoxemia in rats. Methods A rat model of alcoholic fatty liver was established by gradient ethanol gavage method;The liver steatosis,intrahepatic inflammation,liver function tests and serum lipopolysaccharide were determined at 3 and 6 weeks of experiment. Result Significant liver steatosis was observed after 6 weeks of ethanol administration in the model rats;The liver index,liver steatosis scores,inflammation scores in model rats were（3.6±0.2）,（3.0±0.9）and（1.0±0.6）,respectively, significantly higher than those in normal controls[（3.1±0.1）,（0.0±0.0） and（0.0±0.0）,respectively,P<0.05）];Similarly,serum lipopolysaccharide,D-lactate,diamine oxidase and aspertate aminotransferase levels in model rats at 6 months were（1435.6±52.9） pg/ml,（20.7±5.4） mmol/L,（25.5±2.0）U/L and （124.5±13.2） U/L,which were also significantly higher than those in normal controls[（89.9±10.5） pg/ml,（5.0±1.1）mmol/L,（7.4±1.7） U/L and（40.4±15.2） U/L,respectively,P<0.05]. Conclusion A rat model of alcoholic fatty liver accompanied with intestinal endotoxemia can be successfully establish by using gradient ethanol gavage for 6 weeks.

Key words: Alcoholic fatty liver, Intestinal endotoxemia, Permeability of intestinal mucosa, Rats