复方鳖甲软肝片对二乙基亚硝胺所致原发性肝癌大鼠肝组织PCNA和α-SMA表达的影响*

doi:10.3969/j.issn.1672-5069.2018.04.017

摘要/Abstract

摘要： 目的研究复方鳖甲软肝片对二乙基亚硝胺（DEN）所致原发性肝癌大鼠肝癌组织增殖细胞核抗原（PCNA）和平滑肌肌动蛋白（α-SMA）表达的影响。方法将40只SD大鼠随机平均分为对照组、模型组、大剂量和小剂量复方鳖甲软肝片处理组,每组均为10只。应用DEN注射法制备原发性肝癌模型,分别给予复方鳖甲软肝片0.625 g.kg^-1和1.25 g.kg^-1或生理盐水灌胃,连续给药20周。采用免疫组化法检测肝组织PCNA和α-SMA表达。结果大剂量组肝脏质量、肝脏癌变率、肝脏指数和肝表面癌结节数分别为（17.34±1.78）g、10.0%、（3.10±0.72）和（3.16±0.45）个,显著低于模型组【（25.59±2.38） g、100.0%、（4.95±0.58）和（14.14±2.37）个,P<0.05】;小剂量组肝脏质量、肝脏癌变率、肝脏指数和肝表面癌结节数分别为（19.89±1.80）g、30.0%、（3.89±0.81）和（7.38±1.01）个,也显著低于模型组（P<0.05）;大剂量组PCNA和α-SMA相对表达量分别为（5.98±1.12）和（7.96±1.37）,小剂量组分别为（9.23±1.34）和（12.34±2.88）,均分别明显低于模型组【（19.59±2.67）和（25.12±3.19）,P<0.05】。结论复方鳖甲软肝片对DEN所致原发性肝癌大鼠具有较好的防治作用,可能是通过调节肝组织PCNA和α-SMA表达发挥作用的。

关键词: 原发性肝癌, 二乙基亚硝胺, 复方鳖甲软肝片, 增殖细胞核抗原, 平滑肌肌动蛋白, 大鼠

Abstract: Objective To observe the protective effect of Fufangbiejia, a herbal medicine, on diethylnitrosamine (DEN)-induced primary liver cancer (PLC) in rats and its impact on proliferating cell nuclear antigen (PCNA) and α-SMA expression. Methods 40 SD rats were randomly divided into control, model, high and low dose of herbal medicine-intervened groups, with 10 in each. The DEN was used to induce hepatic neoplasmas in rats, and the herbal medicine at dose of 0.625 g·kg^-1 and 1.25 g·kg^-1 or saline for gavage were given for 20 weeks. The PCNA and α-SMA were stained immunohistochemically. Results The liver weights, surface carcinoma nodule numbers, canceralization rates and liver index were （17.34±1.78） g, 10.0%, （3.10±0.72） and （3.16±0.45） in high dose group, significantly lower than in the model group 【（25.59±2.38） g, 100.0%,（4.95±0.58） and （14.14±2.37）,P<0.05】; the liver weights, surface carcinoma nodule numbers, canceralization rates and liver index were （19.89±1.80）g, 30.0%, （3.89±0.81） and （7.38±1.01） in low dose group, also significantly lower than in the model group and(P<0.05);the expression scores of PCNA and α-SMA were （5.98±1.12） and （7.96±1.37） in high dose group,and （9.23±1.34） and （12.34±2.88） in low dose group, both significantly lower than in the model group 【（19.59±2.67） and （25.12±3.19）,P<0.05】. Conclusions Fufangbiejia has a protective effect on DEN-induced primary liver cancer in rats, which might be related to the regulation of hepatic PCNA and α-SMA expression.

Key words: Hepatoma, Diethylnitrosamine, Herbal medicine, PCNA, α-SMA;, Rats

高翔, 尚双艳. 复方鳖甲软肝片对二乙基亚硝胺所致原发性肝癌大鼠肝组织PCNA和α-SMA表达的影响^*[J]. 实用肝脏病杂志, 2018, 21(4): 553-556.

Gao Xiang, Shang Shuangyan. Protective effect of Fufangbiejia, a herbal medicine, on diethylnitrosamine-induced primary liver cancer in rats and its impact on PCNA and α-SMA expression[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(4): 553-556.

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复方鳖甲软肝片对二乙基亚硝胺所致原发性肝癌大鼠肝组织PCNA和α-SMA表达的影响^*

Protective effect of Fufangbiejia, a herbal medicine, on diethylnitrosamine-induced primary liver cancer in rats and its impact on PCNA and α-SMA expression

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