关键词: 丙型肝炎病毒, 树突状细胞, 模拟肽, 干扰素-γ, 肿瘤坏死因子-α, 白介素-10

Abstract: Objective To investigate the immune response and its mechanism of dendritic cells induced by a polypeptide. Methods Using the method of“Bulk”to harvest many DCs. After subcutaneously immunization for ten days，the mice splenocytes were harvested. CD4+T cells were separated from splenocytes by cell sorting technology. Then，CD4+T cells and DC cells were coincubated for a day. The supernatants were detected by cytometric bead array. Result The CD11c+ expression rate of DC cultured in vitro was more than 80%；Compared with control group，the level of TNF-α secreted by CD4+T cell was lower and the levels of IL-5，IL-2 and IFN-γ were higher in experiment group；the levels of IFN-γ，TNF-α，IL-10 and IL-6 secreted by CD8+T cell were increased in experiment group. Conclusion A large number of DCs from mouse bone marrow in vitro exhibites typical morphology of DC，which might be an experimental material for further study on function，markers and antitumor role of DCs in vitro. The 7 peptide could induce cell-mediated immune response through Th1 cells and CD8+T cells.

Key words: Hepatitis C virus, Mimic Peptide, Dendritic cells, IFN-γ, TNF-α, IL-10