慢加急性乙型肝炎肝衰竭患者血清IFN-γ基因多态性分析

doi:10.3969/j.issn.1672-5069.2020.03.020

摘要/Abstract

摘要： 目的探究慢加急性乙型肝炎肝衰竭(HBV-ACLF)患者血清γ-干扰素(IFN-γ)基因多态性分布特点。方法 2017年5月～2019年9月我院收治的HBV-ACLF患者60例,另选择同期健康体检者60例作为对照。采用Taqman探针荧光定量PCR法检测外周血IFN-γ基因多态性位点+874T/A和+2109A/G基因型,采用x²检验和Hardy-Weinberg平衡检验IFN-γ基因SNP位点+874T/A和+2109A/G基因型和等位基因频率分布差异。结果在IFN-γ+874T/A位点,ACLF患者TT基因型为48.3%,显著低于健康人的56.7%,而AA基因型为31.7%,显著高于健康人的13.3%(P＜0.05),在等位基因频率分布方面,ACLF患者T等位基因频率为58.3%,显著低于健康人的71.7%,而A等位基因频率为41.7%,显著高于健康人的28.3%(P＜0.05);在IFN-γ+2109A/G位点,ACLF患者显性等位基因纯合子AA基因型频率为53.3%,显著低于健康人的66.7%,隐性等位基因纯合子GG基因型频率为28.3%,显著高于对照组的10.0%(P＜0.05),在等位基因频率分布方面,ACLF患者A等位基因频率为62.5%,显著低于对照组的78.3%,而G等位基因频率为37.5%,显著高于对照组的21.7%,差异具有统计学意义(P＜0.05)。结论 IFN-γ基因多态性与乙型肝炎患者发生ACLF可能存在某种关系,其中IFN-γ基因+874T/A位点中的A等位基因和+2109A/G位点中的G等位基因可能是患者发生HBV-ACLF的风险等位基因,监测外周血IFN-γ基因多态性对及时准确地预测ACLF的发生可能具有一定的临床意义。

关键词: 慢加急性肝衰竭, 乙型肝炎, γ-干扰素, 基因多态性 , ,

Abstract: Objective The aim of this study was to explore blood interferon-gamma (IFN-γ) gene polymorphism in patients with acute-on-chronic hepatitis B liver failure (HBV-ACLF). Methods 60 patients with HBV-ACLF and 60 healthy persons for physical examination were recruited in our hospital from May 2017 through September 2019, blood genotyping of IFN-γ gene polymorphism sites +874T/A and +2109A/G were performed by Taqman probe quantitative PCR, and the distribution characteristics of genotypes and allele frequencies of the two polymorphism sites were observed. The correlation of the polymorphism sites to the occurrence of ACLF was analyzed by x² test and Hardy-weinberg equilibrium test. Results At IFN-γ+874T/A site, the frequency of TT genotype in patients with HBV-ACLF was 48.3%, significantly lower than 56.7%, while that of AA was 31.7%, significantly higher than 13.3%(P＜0.05) in the healthy persons, and at allele frequencies, the frequency of T allele in patients with HBV-ACLF was 58.3%, significantly lower than 71.7%, while that of A allele was 41.7%, significantly higher than 28.3%(P＜0.05) in the control; at IFN-γ+2109A/G site, the frequency of AA genotype, an Dominant allele homozygote, was 53.3%, significantly lower than 66.7%, while the frequency of GG genotype, an Recessive allele homozygote, was 28.3%, significantly higher than 10.0%(P＜0.05) in the control, and at allele frequency, the frequency of A allele in patients with HBV-ACLF was 62.5%, much lower than 78.3%, while the frequency of G allele was 37.5%, significantly higher than 21.7% (P＜0.05) in healthy persons. Conclusion IFN-γ gene polymorphism is possibly correlated with HBV-ACLF occurrence in patients with hepatitis B, and among which A allele in +874T/A locus and G allele in +2109A/G locus might be the risk gene for HBV-ACLF occurrence. Monitoring IFN-γ gene polymorphism in patients with hepatitis B might be helpful in predicting HBV-ACLF happening.

Key words: Acute-on-chronic liver failure, Hepatitis B, Interferon-γ, Polymorphism of gene

高朋彬,赵晓彦,王德华,秦浩,孟明辉,张建集,闫双缓,郑欢伟. 慢加急性乙型肝炎肝衰竭患者血清IFN-γ基因多态性分析[J]. 实用肝脏病杂志, 2020, 23(3): 380-383.

Gao Pengbin, Zhao Xiaoyan, Wang Dehua, et al.. Blood interferon-gamma gene polymorphism in patients with acute-on-chronic hepatitis B liver failure[J]. Journal of Practical Hepatology, 2020, 23(3): 380-383.

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