香附多糖对牛血清白蛋白诱导的肝纤维化大鼠血清MMP-2、TIMP-2和TGF-β1水平的影响

doi:10.3969/j.issn.1672-5069.2018.01.010

摘要/Abstract

摘要： 目的研究香附多糖对牛血清白蛋白诱导的肝纤维化大鼠血清基质金属蛋白酶2（MMP-2）、组织金属蛋白酶抑制剂-2（TIMP-2）和转化生长因子-β1的影响。方法将60只SD大鼠随机平均分为正常对照组、模型组、秋水仙碱（0.1 mg·kg^-1）、大剂量香附多糖组（200 mg·kg^-1）、中等剂量香附多糖组（100 mg·kg^-1）和小剂量香附多糖组（50 mg·kg^-1）,每组10只。在造模成功后,分别给予生理盐水、秋水仙碱和香附多糖灌胃处理,连续60 d。在末次给药后,采用腹主动脉取血,分离血清,并取肝组织行病理学检查。检测各组大鼠血清肝功能指标和肝纤维化指标,采用酶联免疫分析法检测血清MMP-2、TIMP-2和TGF-β1水平,并观察各组大鼠肝组织病理学变化情况。结果与模型组比较,大剂量、中剂量和小剂量香附多糖组大鼠血清ALT、AST、TP、ALB水平得到显著改善（P<0.05）,血清LN、HA、PC-Ⅲ和Ⅳ-C水平均明显降低（P<0.05）;大、中、小剂量组大鼠血清MMP-2水平分别为（221.58±23.76）ng/mL、（291.12±29.35） ng/mL、（375.14±38.21） ng/mL,TIMP-2水平分别为（161.89±16.12） μg/mL、（180.34± 17.12） μg/mL和（196.56±19.34）μg/mL,TGF-β1水平分别为（206.23±21.45）ng/mL、（251.53±26.10） ng/mL、（302.09±31.21） ng/mL,均显著低于模型组（P<0.05）;肝组织病理学检查结果显示,与模型组比较,大、中、小剂量药物处理组大鼠肝纤维化程度均有明显减轻。结论香附多糖具有较好的保肝及抑制肝纤维化的活性,可能是有效改善了大鼠MMP-2/TIMP-2失衡和降低TGF-β1水平有关。

关键词: 肝纤维化, 香附多糖, 抗肝纤维化, 大鼠

Abstract: Objective To investigate the anti-fibrotic effects of cyperus rotundus polysaccharides and the changes of serum matrix metalloproteinase 2（MMP-2）,tissue metalloproteinase inhibitor-2（TIMP-2） and transforming growth factor-beta1（TGF-β1） in rats with bovine serum albumin（BSA）-induced hepatic fibrosis. Methods 60 Sprague-Dawley rats were randomly divided into normal control group, model group,colchicine group （0.1 mg·kg^-1）,large dose of polysaccharide group （200 mg·kg^-1）,middle dosage group （100 mg·kg^-1） and low-dose polysaccharide group （50 mg·kg^-1）. The rats were intragastrically given cyperus rotundus polysaccharide, colchicine or normal saline for 60 days. After the last administration,the blood was obtained by through abdominal aorta, sera were collected and the liver tissues were taken. Serum levels of MMP-2,TIMP-2 and TGF-β1 were measured by ELISA and the pathological changes of liver tissues were observed in each group. Results Serum levels of ALT,AST,TP and ALB in large dose group,middle dose group and low dose group were significantly decreased（P<0.05）,and serum levels of LN,HA,PC-Ⅲ and Ⅳ-C were significantly reduced as compared with in the model group （P<0.05）;serum MMP-2 levels in the large,middle and low dose groups were （221.58±23.76） ng/mL,（291.12 ± 29.35） ng/mL,and（375.14±38.21） ng/mL,serum TIMP-2 levels were （161.89±16.12） μg/ml,（180.34±17.12） μg/ml,and （266.56±19.34） μg/ml,and serum levels of TGF-β1 were（206.23±21.45） ng/mL,（251.53±26.10） ng/mL,and（302.09±31.21） ng/mL,respectively,all being obviously improved as compared to those in the model group（P<0.05）;histopathological examination results showed that the liver fibrosis of the rats in the large,middle and low dose groups were significantly alleviated as compared with in the model group. Conclusion The cyperus rotundus polysaccharides has a good effect on hepatic fibrosis,and the mechanism by which it works might be related to the restored inbalance of serum MMP-2 and TIMP-2 and decreased TGF-β1 in rats with hepatic fibrosis.

Key words: Liver fibrosis, Cyperus rotundus polysaccharide, Anti-fibrotic effects, Rats

尚双艳, 高翔. 香附多糖对牛血清白蛋白诱导的肝纤维化大鼠血清MMP-2、TIMP-2和TGF-β1水平的影响[J]. 实用肝脏病杂志, 2018, 21(1): 42-45.

Shang Shuangyan, Gao Xiang. Inhibition of hepatic fibrosis by cyperus rotundus polysaccharide in BSA-induced hepatic fibrosis in rats[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(1): 42-45.

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