Abstract: Objective To study the effect of Ganfukang compound，a Chinese traditional herb medicine，on the expression of some regulatory factors of Wnt signaling pathway in hepatic stellate（HSC）-T6 cells. Methods HSC-T6 was dealt with middle concentration of Ganfukang compound. The expression changes of Wnt1，Gsk-3β，β-catenin，cyclinD1 and α-SMA were analysed by RT-PCR and Western-blot. Results Except Gsk-3β，the expression of Wnt1，β-catenin，cyclinD1 and α-SMA were downregulated after treated with Ganfukang compound. Conclusion The anti-fibrosis roles of Ganfukang compound are related to several key regulatory factors in Wnt/β-catenin pathway，which might inhibit the transcription of α-SMA and decrease the production of extracellular matrix.

Key words: Liver fibrosis, Hepatic stellate cell, Chinese herb medicine, Wnt/β-catenin pathway