Abstract: Objective This experiment aimed to investigate effect of Yiqi Huoxue Tongluo compound, herbal medicine, intervention on portal vein recanalization in rats with surgery-induced portal vein thrombosis (PVT). Method 45 rats were randomly divided into experimental (n=40) and control (n=5) group. The PVT model was established by intermittent portal vein ligation and clamping, and monitored by ultrasonography. Rats with PVT were randomly divided into model (n=6), model control (n=6), rivaroxaban-intervened (n=12) and herbal medicine-intervened (n=12) group. Result Ultrasonography showed PVT was successfully established; 2 weeks after intervention, the reperfusion rate in rivaroxaban group was 44.4% (4/9), and it was 90.9% (10/11, P<0.05) in herbal medicine-intervened group; pathologically, vascular intima injury, media edema and thickening, and collagenous fiber adhesion in model, model control and rivaroxaban group, while vascular intima basically intact with slightly thicken media and much less collagenous fiber adhesion in herbal medicine-intervened group; serum D-dimer levels in herbal and rivaroxaban-intervened groups were (170.7±6.7)ng/mL and (176.8±7.3)ng/mL, both significantly lower than [(211.3±12.4)ng/mL, P<0.05] in model or [(205.1±12.7)ng/mL, P<0.05] in model control group. Conclusion A stable rat PVT model is successfully established by using intermittent ligation and clamping. Both rivaroxaban and herbal medicine, Yiqi Huoxue Tongluo Formula are effective in promoting PVT recanalization, which needs further investigation.

Key words: Portal vein thrombosis, Intermittent ligation and clamping, Levaroxaban, Herbal medicine, Yiqi Huoxue Tongluo compound, Rats