Abstract: Objective The aim of this study was to investigate the structure of intestinal flora changes in patients with non-alcoholic fatty liver disease (NAFLD) and diabetes mellitus type 2(T2DM). Methods 58 patients with NAFLD and T2DM, 66 patients with NAFLD and 60 healthy volunteers were encountered in our hospital between June 2018 and July 2020. The intestinal flora structure, the homeostasis model assessment-insulin resistance steady state model insulin resistance (HOMA-IR) and serum tumor necrosis factor-α(TNF-α) and high-sensitivity C-reactive protein levels were detected. Results The fecal phylum of Bacteroides in patients with NAFLD and T2DM was significantly higher than that in patients with NAFLD or in healthy control (P＜0.05), and the phylum of Bacteroides in patients with NAFLD was significantly higher than that in healthy control (P＜0.05); the abundances of fecal Firmicutes, Clostridium tenuis, Anaerobic Streptomyces and Blautella in patients with NAFLD and T2DM were significantly lower than those in patients with NAFLD or in healthy individuals (P＜0.05), and the abundances of Firmicutes, Clostridium tenuis, Anaerobic bacillus, and Blautella in patients with NAFLD were all significantly lower than those in the healthy control (P<0.05); the HOMA-IR index, serum TNF-α and hs-CRP levels in patients with NAFLD and T2DM were (2.8±1.3), (52.3±11.7)ng/mL and (2.3±1.1)mg/L, significantly higher than [(1.6±0.7), (48.7±10.2)ng/mL and (1.6±0.9)mg/L, respectively, P<0.05] in patients with NAFLD or [(1.3±0.3), (33.2±8.4)ng/mL and (1.2±0.4)mg/L, respectively, P<0.05] in healthy individuals. Conclusion The patients with NAFLD and T2DM have insulin resistance and serum inflammatory factor disorders, as well as the structure changes of intestinal flora, which needs further investigation for its clinical implications.

Key words: Non-alcoholic fatty liver disease, Type 2 diabetes mellitus, Intestinal flora, Insulin resistance, Inflammatory factors