慢性HBV感染者外周血白细胞GRP78、CHOP和XBP1 mRNA水平变化*

doi:10.3969/j.issn.1672-5069.2015.02.008

Abstract

Abstract: Objective To investigate the changes of glucose regulated protein 78（GRP78）,a endoplasmic reticulum stress （ERS） related gene,C/ enhancer-binding protein homologous protein （CHOP） and X-box binding protein（XBP） 1 mRNA in peripheral leukocytes in patients with chronic hepatitis B viral infections. Methods The mRNA levels in peripheral leukocytes were measured by real time PCR in 43 individuals with asymptomatic hepatitis B virus carriers,47 patients with chronic hepatitis B,41 with hepatitis B related liver cirrhosis,35 with hepatitis B related hepatocellular carcinoma（HCC） and 40 normal healthy persons. Results The GRP78 mRNA levels in hepatitis B virus carriers,in patients with chronic hepatitis B,liver cirrhosis and hepatocellular carcinoma were higher than that in healthy controls[（0.58±0.53）,（0.76±0.57）,（0.59±0.67） and （0.50±0.64） vs. （0.01±0.61）,P<0.01 for all];the XBP 1 mRNA in hepatitis B virus carriers,in patients with chronic hepatitis B,liver cirrhosis and hepatocellular carcinoma were also higher than that in health controls [（1.07±1.09）,（1.44±1.00）,（1.17±1.69） and （0.90±1.33） vs. （0.01±0.97）,P<0.05 for all];The GRP78 mRNA （0.48±0.59） in patients with serum HBsAg levels of <1500 IU/ml were much lower than in with serum 1500~20000 IU/ml[（0.76±0.56）,P<0.05] or >20000 IU/ml[（0.74±0.56）,P<0.05];The XBP 1 mRNA levels in patients with serum HBV DNA levels of 1×10³ to 10⁵copies/ml was lower than in patients with higher serum HBV DNA load（>1×10⁵copies/ml）[（0.86±1.13） vs. （1.44±1.31）,P<0.05];However,there existed no statistical differences as respect to the three mRNA levels among patients with different serum alanine aminotransferase,nor between patients with normal or abnormal serum bilirubin,nor between HBeAg-positive and HBeAg-negative patients. Conclusion Endoplasmic reticulum stress may beassociated with the disease progression in patients with chronic HBV infection.

Key words: Hepatitis B, Glucose regulated protein 78, C/enhancer-binding protein homologous protein, X-box binding protein 1, Endoplasmic reticulum stress

Pan Gaofeng, Gao Yufeng, Ye Jiaojiao, et al.. Peripheral leukocyte GRP78,CHOP and XBP1 mRNA levels in patients with chronic hepatitis B viral infection[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2015, 18(2): 136-140.

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Peripheral leukocyte GRP78,CHOP and XBP1 mRNA levels in patients with chronic hepatitis B viral infection

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