Abstract: Objective To explore the protective effect of bee venom hemolysin on rats with nonalcoholic fatty liver disease （NAFLD）.Methods Forty male SD rats were randomly divided into control,model,low-dose and high dose of bee venom hemolysin-intervened groups. Rats were fed with high fat to establish the NAFLD models,and the bee venom hemolysin peptide at doses of 10 μg·kg^-1 and 100 μg·kg^-1 or saline were subcutaneously injected daily for 12 weeks. Hepatic expression of nuclear factor-erythroid 2 p45-related factor 2 （Nrf2） and heme oxygenase-1（HO-1） were detected by Western bloting.Results The body weight and liver mass of rats in high dose of bee venom hemolysin-intervened group were（380.2±20.8） g and（10.4±1.3） g,significantly lower than（435.2±22.1） g and （14.3±1.4） g in the model （P<0.05）,serum AST and ALT levels were （88.0±10.4） U/L and （49.3±6.2） U/L,much lower than （159.7±18.9） U/L and （77.7±6.8） U/L in the model （P<0.05）,blood glucose,TC,TG,and LDL-C levels were（8.7±1.8） mmol/L,（1.6±0.2） mmol/L,（0.8±0.1） mmol/L and （0.3±0.1）mmol/L,significantly lower than （18.3±2.4） mmol/L,（2.8±0.3） mmol/L,（1.5±0.2） mmol/L and （0.5±0.1）mmol/L,respectively in the model （P<0.05）,serum superoxide dismutase（SOD）,malondialdehyde（MDA） and glutathione（GSH） levels were （82.1±6.6）U/L,（6.3±0.8）nmol/mL and （8.7±0.9） mmol/L,significantly different as compared to （30.4±5.3）U/L,（13.1±1.6）nmol/mL and（2.3±0.5）mmol/L in the model（P<0.05）,hepatic expression of Nrf2 and HO-1 were （1.4±0.2） and （1.2±0.1）,significantly intensified as compared to（0.3±0.1） and （0.3±0.1） in the model （P<0.05）. Conclusion Bee venom hemolytic peptide might alleviate hepatic steatosis in rats with non-alcoholic fatty liver disease and improve liver function,and the mechanism might be related to the regulation of Nrf2 and HO-1 expression and relieve oxidative stress injury.

Key words: Liver steatosis, Bee venom hemolytic peptide, Nuclear factor-erythroid 2 p45-related factor 2, Heme oxygenase-1, Rats