综合基因表达谱分析与人类肝胰岛素抵抗相关蛋白分子表达研究*

doi:10.3969/j.issn.1672-5069.2018.06.012

Abstract

Abstract: Objective To analyze of differentially expressed genes （DEGs） between diabetic and normal liver tissues and to screen proteins in liver tissues that may be associated with insulin resistance. Methods After download the gene expression profile GSE23343 （including gene expression value from 10 cases of type 2 diabetes mellitus and 7 cases of normal liver） from the GEO Database,and the protein-protein interaction network of DEGs was constructed by DEGs expression profiling and functional pathway enrichment analysis. Results After analysis of the microarray data,928 DEGs were found to be significantly up-regulated（P<; 0.01）. Functional pathway enrichment analysis showed that most of the differential genes were enriched in cellular and metabolic processes,and KEGG pathway enrichment showed that DEGs were mainly focused on signal transduction and tumor-associated pathways. Five key protein molecules,e.g. MDM2,PCNA,CAV1,PIK3R1,and NR3C1,were identified by protein-protein interaction network. Conclusion The protein molecules related to the formation of insulin resistance in human liver tissues are systematically selected,which might provide us the basis for the further study of the mechanism of hepatic insulin resistance and for finding new targets of hypoglycemic medicine.

Key words: Nonalcoholic fatty liver diseases, Insulin resistance, Differentially expressed genes, Protein-protein interaction network

Huang Faguang, Li Xiaocheng, Gong Jianping, et al.. Research on insulin resistance related protein molecules in human liver tissues by comprehensive gene expression profiling analysis[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(6): 872-876.

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Research on insulin resistance related protein molecules in human liver tissues by comprehensive gene expression profiling analysis

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