39例成人急性乙型肝炎患者血清HBV基因变异检测及其临床意义探讨*

doi:10.3969/j.issn.1672-5069.2016.05.004

Abstract

Abstract: Objective To investigate the prevalent viral genotypes and possible gene mutation in adults with acute hepatitis B（AHB）. Methods Serum samples of 39 patients with AHB admitted in our hospital were collected between December 2012 and January 2014. Viral genotypes and sequences of P region,S region,Pre C region and basic core promoter（BCP） were detected by direct sequencing and parallel allele specific sequencing （PASS）. Results 35 out of 39 patients with AHB were sequenced successfully. Genotype distribution in these 35 cases was as follows. Genotype B accounted for 36.4% （12/35）,genotype C for 57.6% （21/35）,and genotype D for 6.0%（2/35）;A181S drug resistance mutation was found in one patient by both sequencing methods,while S132F and W172C mutations in S region as well as G1896A and T1758C mutations in PreC/BCP regions were noticed by direct sequencing in two different cases,respectively. Conclusions Viral genotype distribution of common genotype C and B in patients with AHB is not different in patients with CHB. Patients infected with P region,S region or PreC/BCP region mutants might have similar prognosis as patients infected with wild HBV.

Key words: Acute hepatitis B, Genotype, Mutation

Du Xiaofei, Ma Lina, Liu Yali et al.. Hepatitis B viral genotypes and gene mutations in 39 adult patients with acute hepatitis B[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(5): 524-527.

References

[1] GBD 2013 Mortality and Causes of Death Collaborators. Global, regional,and national age-sex specific all-cause and cause-specific mortality for 240 causes of death,1990-2013:a systematic analysis for the Global Burden of Disease Study 2013. Lancet,2015,385：117-171.
[2] Trepo C,Chan HL,Lok A. Hepatitis B virus infection. Lancet,2014,384：2053-2063 .
[3] Xu Z,Liu Y,Xu T,et al. Acute hepatitis B infection associated with drug-resistant hepatitis B virus. J Clin Virol,2010,48:270-274.
[4] Liu Y,Zhong Y,Zou Z,et al. Features and clinical implications of hepatitis B virus genotypes and mutations in basal core promoter/precore region in 507 Chinese patients with acute and chronic hepatitis B. J Clin Virol,2010,47:243-247.
[5] 赵琳,黄玲,王晶慧. 240例初治乙型肝炎患者的流行病学特征. 肝脏,2011,16:442-445.
[6] Thibault V,Aubron-Olivier C,Agut H,et al. Primary infection with a lamivudine-resistant hepatitis B virus. Aids,2002,16:131-133.
[7] 中华医学会传染病与寄生虫病学分会、肝病学分会联合修订. 病毒性肝炎防治方案. 中华肝脏病杂志,2000,8:5-10.
[8] Ozasa A,Tanaka Y,Orito E,et al. Influence of genotypes and precore mutations on fulminant or chronic outcome of acute hepatitis B virus infection. Hepatology,2006,44:326-334.
[9] Matsuura K,Tanaka Y,Hige S,et al. Distribution of hepatitis B virus genotypes among patients with chronic infection in Japan shifting toward an increase of genotype A. J Clin Microbiol,2009,47:1476-1483.
[10] Yotsuyanagi H, Ito K,Yamada N,et al. High levels of hepatitis B virus after the onset of disease lead to chronic infection in patients with acute hepatitis B. Clin Infect Dis,2013,57:935-942.
[11] Yano K,Tamada Y,Yatsuhashi H,et al. Dynamic epidemiology of acute viral hepatitis in Japan. Intervirology,2010,53:70-75.
[12] Ito K,Yotsuyanagi H,Yatsuhashi H,et al. Risk factors for long term persistence of serum hepatitis B surface antigen following acute hepatitis B virus infection in Japanese adults. Hepatology,2014,59:89-97.
[13] Li HM,Wang JQ,Wang R,et al. Hepatitis B virus genotypes and genome characteristics in China. World J Gastroenterol,2015,21（21）:6684-6697.
[14] Chattopadhyay S,Das BC,Hussain Z,et al. Hepatitis B virus genotypes in acute and fulminant hepatitis patients from north India using two different molecular genotyping approaches. Hepatol Res,2006,35:79-82.
[15] Suzuki Y,Kobayashi M,Ikeda K,et al. Persistence of acute infection with hepatitis B virus genotype A and treatment in Japan. J Med Virol,2005,76:33-39.
[16] Hayashi K,Katano Y,Ishigami M,et al. Prevalence and clinical characterization of patients with acute hepatitis B induced by lamivudine resistant strains. J Gastroenterol Hepatol,2010,25:745-749.
[17] Baxa DM,Thekdi AD,Golembieski A,et al. Evaluation of anti-HBV drug resistant mutations among patients with acute symptomatic hepatitis B in the United States. J Hepatol,2013,58:212-216.
[18] Morando F,Rosi S,Fasolato S, et al. Severe acute hepatitis B in a treatment naive patient with antiviral drug resistant mutations in the polymerase gene. J Med Virol,2013,85:210-213.
[19] Clements CJ,Coghlan B,Creati M,et al. Global control of hepatitis B virus:does treatment-induced antigenic change affect immunization. Bull World Health Organ,2010,88:66-73.
[20] Sainokami S,Abe K,Sato A,et al. Initial load of hepatitis B virus（HBV）,its changing profile,and precore/core promoter mutations correlate with the severity and outcome of acute HBV infection. J Gastroenterol,2007,42:241-249.

Hepatitis B viral genotypes and gene mutations in 39 adult patients with acute hepatitis B

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Deng Haohui, Li Xiaoqiang, Gao Hongbo, et al. Comparison of HCV genotypes in patients with HIV/HCV co-infection and HCV mono-infection in Guangzhou [J]. Journal of Practical Hepatology, 2019, 22(5): 656-659.
[2]	Zhang Huanhuan, Yang Huan, Shen Silan, et al.. Distribution of HBV genotypes and drug-resistant mutations in patients with chronic hepatitis B [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(1): 41-44.
[3]	Hou Chunyang, Ma Yumei, Li Fangzhi.. Clinical efficacy of peg-IFNα and ribavirin combination in treating patients with chronic hepatitis C and genotype one and six infection [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(1): 53-56.
[4]	Zhao Peng, Wang Lei.. Comparison of therapeutic efficacy of pegylated interferon-α in treatment of patients with different hepatitis C viral genotype infection [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(1): 57-60.
[5]	Tan Guolei, Sun Mei, Wang Jianfang, et al. Polymorphisms of peripheral blood IL-28B gene in patients of Han nationality with hepatitis B virus infection [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(4): 561-564.
[6]	Wu Xiao, Wei Shaofeng, Xu Nan, et al.. Meta-analysis on distribution of hepatitis B virus genotypes and related clinical outcomes in China [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2017, 20(3): 271-275.
[7]	Deng Jiazheng, Shen Yang, Ling Jing, Deng Hong, Le Yonghong, Zhuang Xiaofang. Transforming growth factor-α-435G/A gene polymorphism in patients with nonalcoholic fatty liver diseases [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2017, 20(1): 65-68.
[8]	Shi Ping，Shen Jiandong， Liu Yao，et al.. Mutation of uridine diphosphate glucuronosyltransferase in three patients with Crigler-Najjar syndrome type II [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(6): 654-656.
[9]	Deng Haohui, Xu Min, Gao Hongbo, et al.. Hepatitis B virus subgenotypes in 424 patients with hepatitis B,hepatic failure, liver cirrhosis and hepatocellular carcinoma in Guangzhou [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(5): 528-531.
[10]	Zhang Lin, Dou Zhihua, Qin Gang, et al.. Efficacy and safety of boceprevir combined with peginterferon alfa and ribavirin in treatment of hepatitis C patients with genotype 1 infection: A Meta-analysis [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(5): 544-548.
[11]	Tang Weiliang, Xie Qing. Hepatitis C virus genotype 3 infection [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(4): 394-397.
[12]	Liu Feifei, Zhang Haiyue, Zhang Qian, et al. Clinical features of patients with chronic hepatitis C with concomitant diabetes type 2 and impact of insulin resistance on efficacy of antiviral therapy: a Meta analysis [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(4): 436-440.
[13]	Hao Jianhong, Hu Yiling, Lin Aiqing. Hepatitis B viral gene mutation in patients with hepatitis B [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(4): 473-475.
[14]	Zhang Xiehua, Lin Aiqing, Hu Yiling, et al. Hepatitis B viral gene mutation in 102 patients with hepatitis B viral infection [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(4): 480-481.
[15]	Su Minghua, Jiang Jianning. Hepatitis B virus genotype 6:Epidemiology and treatment of patients [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(4): 497-499.