Abstract: Objective To investigate the distribution of HCV genotypes between patients with HIV/HCV co-infection and with HCV mono-infection in Guangzhou. Methods A total of 233 patients with chronic hepatitis C were enrolled in this study,including 95 patients with HIV/HCV co-infection and 138 patients with HCV mono-infection. The HCV core and NS5B gene fragments were used for genotyping by using Illumina Miseq platform PE300 model,and for the samples which the NGS data contained mixed HCV infection（more than 1% reads mapping to other genotypes）,the NS5A genotypes specific primers were used to validate the mixed HCV infection. The data was compared by x2 test,fisher’s exact test,t-Student and Mann-Whitney U test. Results The percentage of intravenous drug users（IDUs） in patients with HIV/HCV co-infection was significantly higher than in patients with HCV mono-infection（77.7% vs. 19.6%,P<0.001）,and the percentage of blood transfusion history in patients with HCV mono-infection was much higher than in those with HIV/HCV co-infection（48.6% vs. 3.2%,P<0.001）;the average reads of HCV core gene in patients with HIV/HCV co-infection were（15456±6689） reads,no significantly different as compared to（14323±5321） reads in patients with HCV mono-infection,and th e average reads of HCV NS5B gene in patients with HIV/HCV co-infection were（16432±3467） reads,also no significantly different compared to（17611±5632） reads in patients with HCV mono-infection（P>0.05）;228 patients （97.9%） in our series were infected with single HCV genotypes,and HCV genotype 6a and 3b infections were more prevalent in patients with HIV/HCV co-infection than in with HCV mono-infection （47.4% vs. 26.8%,P=0.001 and 13.7% vs. 3.6%,respectively,P=0.005） and HCV genotype 1b infection was more prevalent in patients with HCV mono-infection than in patients with HIV/HCV co-infection （59.4% vs. 20.0%,P<0.001）;5 patients （2.1%） were found with mix HCV genotype infection,including 4 patients with HIV/HCV co-infection and 1 patient with HCV mono-infection. Conclusion The prevalence of infected HCV genotypes in patients with HIV/HCV co-infection and in with HCV mono-infection might be different,which worth further investigation.

Key words: Hepatitis C, AIDS, HCV genotype, Next-generation sequencing