Abstract: Objective To analyzed the peripheral blood IL-6 174C / G genotype and allele frequencies for the investigation of the peripheral blood IL-6 gene polymorphism in patients with type 1 diabetes mellitus （T1DM） complicated by chronic cholecystitis.Methods 110 patients with T1DM complicated by chronic cholecystitis and 120 healthy individuals were included in this study. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was applied to the genetic polymorphism detection，and the genotype and allele frequencies were calculated. The clinical data and blood biochemistry index were obtained by questionnaires and routine detection. SPSS 17.0 statistical package was used for statistical analysis.Results The age and gender in the two groups were comparable，and the body mass index（BMI） in patients with T1DM complicated by chronic cholecystitis was （28.15±3.98） kg/m2，much higher than （26.86±6.52） kg/m2 in the controls（P<0.05）；the prevalence of blood hypertension in patients with T1DM complicated by chronic cholecystitis was 59.09%，much higher than 32.50% in the control group（P<0.01）；family history of diabetes in the former accounted for 54.55%，much higher than 10.83% in the controls（P<0.01）；the smoking（13.64%） and alcohol consumption（19.09%） in patients with T1DM complicated by chronic cholecystitis were much higher than 11.67% and 8.33%（P＜0.01） in the control；the peripheral blood IL-6 174C/G gene CC genotype in patients was 40.91%，much higher than 28.33%（P＜0.05），while GG genotype was 7.27%，much lower than 19.17% in the control （P＜0.05） and C allele frequencies was 52.73%，much higher than 15.00% in the control（P＜0.01）；the univariate analysis showed that carrying IL-6 174 C allele 【（OR=1.357（1.037～1.776），P＜0.05】，hypertension【OR=1.324（1.187～1.476），P＜0.01】，obesity 【OR=1.165（1.087～1.249） and alcohol consumption [OR=1.113（1.046～1.184），P＜0.01] were the risk factors for patients with T1DM having chronic cholecystitis；the multivariate Logistic analysis showed that carrying IL-6 174 C allele 【OR=1.258（1.119～1.413）】，hypertension【1.225（1.032～1.454）】，obesity 【1.167（1.056～1.289），P＜0.01】 and alcohol consumption 【1.111（1.006～1.228），P＜0.05】 were the independent factors for patients with TIDM having chronic cholecystitis.Conclusion IL-6-174 C/G polymorphism is correlated with the susceptibility of chronic cholecystitis in patients with T1DM.

Key words: Chronic cholecystitis, Type 1 diabetes mellitus, Interleukin-6, Gene polymorphism