Abstract: To explore the relationship between cyp2e-3 gene expression and alcoholic liver disease. Methods In the present study,we recruited 126 patients with alcohol liver disease（ALD） in our hospital from February 2013 to February 2015,and the patients were divided into group A（n=40,with the disease history less than one year）,group B（n=43,with the disease history one to three years）,and group C（n=43,with the disease history greater than three years）. We selected 121 healthy subjects at the same period for control. The hepatic cyp2e-3 mRNA was detected by quantitative PCR,serum cyp2e-3 levels were assayed by ELISA,and hepatic cyp2e-3 expression were detected by Western-blotting or stained immunohistochemistry. Results The hepatic cyp2e-3 mRNA levels in patients with ALD were（34.8±1.7）,much higher than【（6.6±0.7,P<0.05】 in healthy persons;serum cyp2e-3 levels in patients with ALD were higher than in control too;Western-blotting and SP detection found that cyp2e-3 expression in liver tissues in group A,group B,and group C were much stronger than in healthy persons as no cyp2e-3 expression were found in the latter hepatic tissues. Conclusion The activated expression of cyp2e-3 gene in liver tissues in patients with ALD might play a role in the occurrence of liver disease,and it warrants further study.

Key words: Alcoholic liver disease, cyp2e-3 gene, Expression