胆总管结扎诱导大鼠肝肺综合征模型的建立初步研究

doi:10.3969/j.issn.1672-5069.2021.02.007

实用肝脏病杂志 ›› 2021, Vol. 24 ›› Issue (2): 176-179.doi: 10.3969/j.issn.1672-5069.2021.02.007

胆总管结扎诱导大鼠肝肺综合征模型的建立初步研究

荣海芳, 王立国

150000 哈尔滨市哈尔滨市第一医院呼吸内科(荣海芳);
哈尔滨医科大学附属第四医院消化内科(王立国)

收稿日期:2020-05-03 出版日期:2021-03-10 发布日期:2021-04-30
通讯作者: 王立国,E-mail:wangliguo8314000@sina.com
作者简介:荣海芳,女,40岁,医学硕士,主治医师。E-mail:147021955@qq.com
基金资助:
黑龙江省卫生与计生委科研课题(编号:2016-127)

Establishment of hepatopulmonary syndrome induced by common bile duct ligation in rats

Rong Haifang, Wang Liguo

Department of Respiratory Diseases, First Hospital, Harbin 150000,Heilongjiang Province, China

Received:2020-05-03 Online:2021-03-10 Published:2021-04-30

摘要/Abstract

摘要： 目的探讨胆总管结扎术诱导大鼠肝肺综合征(HPS)模型的建立。方法 40只SD大鼠被随机分为实验组30只和对照组(假手术组)10只,采用胆总管结扎术建立肝硬化和HPS模型。使用血气分析仪测定PaO₂、PaCO₂和pH。结果在术后1 w,腹部超声检查显示实验组肝脏回声增粗,胆总管轻度扩张。在术后2 w,肝脏被膜不平整,胆总管显著增粗。在术后4～6 w,肝脏被膜不平整,部分表面可见小结节形成,胆总管显著增宽,并可见腹腔积液;在实验6 w,实验组 PaO₂为(65.5±8.2)mmHg,显著低于对照组【(95.5±4.5)mmHg,P<0.05】,血清一氧化氮(NO)浓度为(64.1±4.5)μmol/L,显著高于对照组【(32.8±4.3)μmol/L,P<0.05】,提示HPS造模成功;组织病理学检查显示实验组动物可见肝脏炎症细胞浸润,纤维条索和部分假小叶形成以及肺脏组织见肺微血管显著扩张。结论采用胆总管结扎术可建立大鼠HPS模型,具有造模周期短、稳定性高、无毒等优势。

关键词: 肝肺综合征, 胆总管结扎术, 大鼠

Abstract: Objective To establish a hepatopulmonary syndrome (HPS) model in rats by ligation of common bile duct.Methods 40 SD rats were randomly divided into experimental (n=30) and control group (n=10), and the rats had operation of common bile duct ligation or sham-operated. Two rats in experimental group and control group were sacrificed at 1, 2, 4, 5, 6 weeks after operation, respectively. PaO₂, PaCO₂ and pH were detected by blood gas analysis.Results At one week, the results of abdominal ultrasonography showed that the echo of the livers was coarse, and the bile duct was slightly dilated; at two weeks, the bile duct was obviously enlarged, and at four to six weeks, the surface of the liver was not flat in the experimental group; the histopathological examination showed that the infiltration of inflammatory cells, the formation of fibrous cords and some pseudolobules in liver tissues, and the dilatation of pulmonary microvessels in the lung tissues were observed; At the end of six week experiment, blood gas analysis showed that PaO₂ was (65.5±8.2)mmHg in the experimental group, significantly lower than【(95.5±4.5)mmHg,P<0.05】in the control, serum nitric oxide level in the experimental group was(64.1±4.5)μmol/L, significantly higher than【(32.8±4.3)μmol/L,P<0.05】 in the control.Conclusion The model of hepatopulmonary syndrome is successfully established by ligation of common bile duct in our experiment, which has the advantages of short model formation, high stability, and no toxicity.

Key words: Hepatopulmonary syndrome, Common bile duct ligation, Rats

荣海芳, 王立国. 胆总管结扎诱导大鼠肝肺综合征模型的建立初步研究[J]. 实用肝脏病杂志, 2021, 24(2): 176-179.

Rong Haifang, Wang Liguo. Establishment of hepatopulmonary syndrome induced by common bile duct ligation in rats[J]. Journal of Practical Hepatology, 2021, 24(2): 176-179.

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胆总管结扎诱导大鼠肝肺综合征模型的建立初步研究

Establishment of hepatopulmonary syndrome induced by common bile duct ligation in rats

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