白藜芦醇对部分肝切除术大鼠肝再生和肝组织NK细胞活性的影响*

doi:10.3969/j.issn.1672-5069.2020.06.007

摘要/Abstract

摘要： 目的探讨白藜芦醇(RES)对肝部分切除大鼠肝组织自然杀伤(NK)细胞活性和肝再生的影响。方法随机将90只SD大鼠分为对照组、小剂量白藜芦醇和大剂量白藜芦醇处理组,各组30只,分别经尾静脉注射生理盐水、白藜芦醇10 g.kg^-1·d^-1和20 g.kg^-1·d^-1,连续5 d,然后行70%肝脏切除术。检测肝脏再生指数,使用流式细胞术测定肝组织NK细胞百分率,采用⁵¹Cr释放法测定NK细胞杀伤活性,采用蛋白印迹法检测肝组织增殖细胞核抗原(PCNA)和CyclinD1蛋白表达。结果在24 h和48 h,大剂量白藜芦醇处理组大鼠肝脏再生指数分别为(1.89±0.04)和(2.45±0.07),显著大于小剂量白藜芦醇处理组【(1.59±0.06)和(2.12±0.05),P<0.05】或对照组【(1.43±0.03)和(1.92±0.05),P<0.05】;大剂量白藜芦醇处理组大鼠NK细胞百分率分别为(24.62±1.36)%和(25.47±1.19)%,显著高于小剂量白藜芦醇处理组【(22.21±1.98)%和(22.36±1.78)%,P<0.05】或对照组【(17.36±1.78)%和(18.65±1.69)%,P<0.05】;大剂量白藜芦醇处理组大鼠NK细胞杀伤活力分别为(48.48±2.69)%和(49.01±2.78)%,显著高于小剂量白藜芦醇处理组【(41.88±2.65)%和(42.32±2.58)%,P<0.05】或对照组【(28.32±2.36)%和(30.12±2.36)%,P<0.05】;大剂量白藜芦醇处理组大鼠肝组织PCNA和CyclinD1蛋白相对表达量显著强于小剂量白藜芦醇处理组和对照组,差异有统计学意义(P<0.05)。结论 RES可以促进肝部分切除术大鼠肝再生,可能与增强了NK细胞活性和相关蛋白表达有关。

关键词: 肝部分切除术, 白藜芦醇, 自然杀伤细胞, 肝再生, 大鼠

Abstract: Objective To explore the effects of resveratrol (RES) on liver regeneration (LR) and liver tissue naturacl killer (NK) cell cytoxicity in rats with partial hepatectomy. Methods 90 SD rats were randomly divided into control, low-dose of RES-intervened and high-dose of RES-intervened group, with 30 rats in each group, receiving normal saline, or RES at dose of 10 g.kg^-1·d^-1 or RES at dose of 20 g.kg^-1·d^-1 for five days. The 70% partial hepatectomy model was established thereafter. At 24 h and 48 h after surgery, 15 rats in each group were sacrificed. LR index was detected. The percentage of liver tissue NK cells was determined by flow cytometry. The cytoxicity of liver NK cells was determined by ⁵¹Cr release method and the Western blotting was applied to detect expression of PCNA and CyclinD1 protein. Results At 24 h and 48 h, the LR indexes in high-dose of RES group were (1.89±0.04) and (2.45±0.07), which were significantly higher than those in low-dose of RES group [(1.59±0.06) and (2.12±0.05), P<0.05] or in control group [(1.43±0.03) and (1.92±0.05), P<0.05]; the percentages of NK cells were (24.62±1.36)% and (25.47±1.19)%, which were significantly higher than those in low-dose of RES group [(22.21±1.98)% and (22.36±1.78)%, P<0.05] or in control group [(17.36±1.78)% and (18.65±1.69)%, P<0.05]; the cytoxicity activities of NK cells were (48.48±2.69) % and (49.01±2.78)%, which were significantly higher than those in low-dose of RES group [(41.88±2.65)% and (42.32±2.58)%, P<0.05] or in control group [(28.32±2.36)% and (30.12±2.36)%, P<0.05]; the relative expression ofhepatic PCNA and CyclinD1 protein were significantly higher than those in low-dose of RES group or in the control group (P<0.05). Conclusion RES could promote NK cell proliferation and LR in rats after partial hepatectomy.

Key words: Partial hepatectomy, Resveratrol, Natural killer cell, Liver regeneration, Rats

李伟, 郭春霞, 刘苏南. 白藜芦醇对部分肝切除术大鼠肝再生和肝组织NK细胞活性的影响^*[J]. 实用肝脏病杂志, 2020, 23(6): 785-788.

Li Wei, Guo Chunxia, Liu Sunan. Increasedliver regeneration and liver tissue NK cell cytoxicity by resveratrol stimulus in rats with partial hepatectomy[J]. Journal of Practical Hepatology, 2020, 23(6): 785-788.

参考文献

[1] Ding C , Fu X , Zhou Y , et al. Disease burden of liver cancer in China from 1997 to 2016: an observational study based on the Global Burden of Diseases. Bmj Open, 2019, 9(4):87-92.
[2] Marongiu F, Marongiu M, Contini A, et al. Hyperplasia vs hypertrophy in tissue regeneration after extensive liver resection . World J Gastroenterol, 2017, 23(10):1764-1770.
[3] Theodotou M, Fokianos K, Mouzouridou A, et al. The effect of resveratrol on hypertension: A clinical trial. Exp Thera Med, 2016, 13(1):295-301.
[4] Rui ZH, Jia JD, Yao ZQ, et al. Antioxidative, anti-inflammatory and hepatoprotective effects of resveratrol on oxidative stress-induced liver damage in tilapia (Oreochromis niloticus). Compar Biochem Physiol Toxicol Pharmacol,2019,215(1):56-66.
[5] Ma Z, Zhang Y, Li Q, et al. Resveratrol improves alcoholic fatty liver disease by downregulating HIF-1α expression and mitochondrial ROS production. Plos One, 2017, 30(8):1103-1114.
[6] Bishayee A , Darvesh A S , Politis T , et al. Resveratrol and liver disease: frombench to bedside and community . Liver Int, 2010, 30(8):1103-1114.
[7] Gershbein LL, Elias H. Observations on the anatomy of the rat liver. Anat Rec, 1954,120(1):85-98.
[8] Haijing B, Jianlin HB. Comprehensive analysis of lncRNA-miRNA-mRNA during proliferative phase of rat liver regeneration. J Cell Physiol, 2019,234(10):18897-18905.
[9] Nederby L, Jakobsen A , Hokland M , et al. Quantification of NK cell activity using whole blood: Methodological aspects of a new test. J Immunol Meth, 2018, 458(5):21-25.
[10] Sanaa E, Seada L S. Quantitation of bcl-2 protein in bladder cancer tissue by enzyme immunoassay: comparison with Western blot and immunohistochemistry. Clin Chem, 2020,44 (7):1423-1429.
[11] Haldrup D , Sara Heebøll, Thomsen K L , et al. Preserved liver regeneration capacity after partial hepatectomy in rats with non-alcoholic steatohepatitis. World J Hepatol, 2018, 10(1):8-21.
[12] Kanazawa H , Obara H , Yoshikawa R , et al. Impact ofmachine perfusion on sinusoid microcirculation of liver graft donated after cardiac death. J Sur Res, 2019, 245:410-419.
[13] Lee JH , Baek SY , Jang EJ , et al. Oxyresveratrol ameliorates nonalcoholic fatty liver disease by regulating hepatic lipogenesis and fatty acid oxidation through liver kinase B1 and AMP-activated protein kinase. Chem Biol Interac, 2018,289(1):68-74.
[14] Jing LG , Yang L , Yanhong S , et al. Humanized mice reveal an essential role for human hepatocytes in the development of the liver immune system. Cell Death Dis, 2018, 9(6):669-690.
[15] Gao JL,Yang L , Yanhong S , et al. Humanized mice reveal an essential role for human hepatocytes in the development of the liver immune system. Cell Death Dis, 2018, 9(6):667-668.
[16] Rita EA , Sara C , Matteo B . Immunecheckpoint-mediated interactions between cancer and immune cells in prostate adenocarcinoma and melanoma. Front Immunol, 2018, 9:1786-1791.
[17] Ilaria H, Roato T, Massimo SH, et al. The uncovered role of immune cells and NK cells in the regulation of bone metastasis. Front Endocrinol, 2019,7(10):145-151.
[18] Vari F , Arpon D , Keane C , et al. Immune evasion via PD-1/PD-L1 on NK-cells and monocyte/macrophages is more prominent in Hodgkin lymphoma than DLBCL. Blood, 2018,131(16):1809-1819..
[19] Swarnalatha Y , Vidhya V G , Murugan A . Isochamanetin is aselective inhibitor for cyclin D1 in SKOV3 cell lines. Nutrit Cancer, 2019,2(5):1-11.
[20] Wu W , Wu Q , Liu X . Chronic activation of FXR induced liver growth with tissue-specific targeting Cyclin D1. Cell cycle, 2019, 18(15):22-25.
[21] Swarnalatha Y , Vidhya V G , Murugan A . Isochamanetin is a Selective Inhibitor for CyclinD1 in SKOV3 Cell Lines. Nutrition Can, 2019,18(12):1-11.
[22] Albrahim T , Binobead M A . Roles of moringa oleifera leaf extract in improving the impact of high dietary intake of monosodium glutamate-induced liver toxicity, oxidative stress, genotoxicity, DNA damage, and PCNA alterations in male rats. Oxid Med Cell Longev, 2018, 2018(3):1-11.

白藜芦醇对部分肝切除术大鼠肝再生和肝组织NK细胞活性的影响^*

Increasedliver regeneration and liver tissue NK cell cytoxicity by resveratrol stimulus in rats with partial hepatectomy

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