红景天苷对原代培养脂肪变性大鼠肝细胞细胞色素P4502E1蛋白表达的抑制作用*

doi:10.3969/j.issn.1672-5069.2015.02.013

实用肝脏病杂志 ›› 2015, Vol. 18 ›› Issue (3): 270-273.doi: 10.3969/j.issn.1672-5069.2015.02.013

红景天苷对原代培养脂肪变性大鼠肝细胞细胞色素P4502E1蛋白表达的抑制作用^*

田丽, 尹玲, 关莉莉, 戴宁

116011 辽宁省大连市大连医科大学附属第一医院消化内科（田丽,尹玲,戴宁）; 消化生理实验室（关莉莉）

收稿日期:2014-12-01 出版日期:2015-05-10 发布日期:2016-02-19
通讯作者: 戴宁, E-mail: dainingdn@163.com E-mail:dltianli@126.com
作者简介:田丽,女,26岁,医学硕士。主要从事慢性肝病防治研究。E-mail:dltianli@126.com
基金资助:
辽宁省教育厅科技攻关计划项目（2008115）

Inhibitory effect of salidroside on cytochrome P4502E1 protein expression in primary-cultured rat hepatocytes with steatosis

Tian Li, Yin Ling, Guan Lili, et al.

First Affiliated Hospital,Dalian Medical University, Dalian 116011,Liaoning Province,China

Received:2014-12-01 Online:2015-05-10 Published:2016-02-19

摘要/Abstract

摘要： 目的研究红景天苷对原代培养大鼠脂肪变性肝细胞细胞色素P4502E1（CYP2E1）蛋白表达的抑制作用。方法体外分离、原代培养SD大鼠肝细胞,选取培养48 h的大鼠肝细胞,加入0.25 mmol/L棕榈酸诱导肝细胞脂肪变性,同步加入150 μg/ml的红景天苷继续培养24 h,检测培养肝细胞上清液ALT、AST、丙二醛（MDA）和肝细胞甘油三酯（TG）含量的变化。采用油红O染色观察肝细胞脂滴沉积,采用蛋白质印迹法检测CYP2E1蛋白表达的变化。结果以0.25 mmol/L棕榈酸和150 μg/ml红景天苷同步培养细胞24 h,红景天苷干预组细胞培养上清ALT[（11.3±1.0） U/L、AST（3.7±0.5） U/L、MDA（1.7±0.2） nmol/ml和TG（105.7±16.8） μg/mg protein]均显著低于棕榈酸模型组[ALT（13.5±1.2） U/L,P<0.05;AST（5.9±0.7） U/L,P<0.05;MDA（4.2±0.9） nmol/ml,P<0.01;TG（268.3±25.8） μg/mg protein,P<0.01];油红O染色显示红景天苷处理肝细胞脂滴明显减少,蛋白质印迹法显示红景天苷能有效抑制脂肪变性肝细胞CYP2E1蛋白表达的上调。结论红景天苷抑制细胞CYP2E1蛋白表达可能是其防治肝细胞脂肪变性的主要机制之一。

关键词: 脂肪肝, 红景天苷, 细胞色素P4502E1, 肝细胞, 氧应激

Abstract: Objective To investigate the inhibitory effect of salidroside on cytochrome P4502E1 （CYP2E1） protein expression in primary-cultured rat hepatocytes with steatosis. Methods The hepatocytes of SD rats were separated and primary-cultured for 48 h. Hepatocytes were then exposed to 0.25 mmol/L palmitic acid incubation for 24 h in the presence or absence of 150 μg/ml salidroside. ALT,AST and malonaldehyde （MDA） in the culture supernatants and triglyceride （TG） contents in the hepatocytes were tested. Lipid accumulation was estimated by oil red-O staining. CYP2E1 protein expression in the hepatocytes was examined by Western blot. Results When compared to palmitic acid group,salidroside decreased ALT[（13.5±1.2） U/L vs.（11.3±1.0） U/L,P<0.05],AST [（5.9±0.7） U/L vs. （3.7±0.5） U/L,P<0.05],MDA[（4.2±0.9） nmol/ml vs. （1.7±0.2） nmol/ml,P<0.01] in the supernatants and TG content in the hepatocytes [（268.3±25.8） μg/mg protein vs. （105.7±16.8） μg/mg protein,P<0.01] induced by palmitic acid;Salidroside attenuated severe accumulation of fat drops in the hepatocytes, and suppressed CYP2E1 protein expression up-regulation in hepatocytes with steatosis. Conclusions Salidroside prevents hepatocyte steatosis via suppression of CYP2E.

Key words: Steatosis, Salidroside, Cytochrome P4502E1, Hepatocytes, Oxidative stress

田丽, 尹玲, 关莉莉, 戴宁. 红景天苷对原代培养脂肪变性大鼠肝细胞细胞色素P4502E1蛋白表达的抑制作用^*[J]. 实用肝脏病杂志, 2015, 18(3): 270-273.

Tian Li, Yin Ling, Guan Lili, et al.. Inhibitory effect of salidroside on cytochrome P4502E1 protein expression in primary-cultured rat hepatocytes with steatosis[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2015, 18(3): 270-273.

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红景天苷对原代培养脂肪变性大鼠肝细胞细胞色素P4502E1蛋白表达的抑制作用^*

Inhibitory effect of salidroside on cytochrome P4502E1 protein expression in primary-cultured rat hepatocytes with steatosis

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