硫代乙酰胺诱导的急性肝损伤大鼠肝组织Norrin/Frizzled-4表达的变化*

doi:10.3969/j.issn.1672-5069.2018.02.008

实用肝脏病杂志 ›› 2018, Vol. 21 ›› Issue (2): 187-190.doi: 10.3969/j.issn.1672-5069.2018.02.008

硫代乙酰胺诱导的急性肝损伤大鼠肝组织Norrin/Frizzled-4表达的变化*

胡建鹏, 宋正己, 李玉莲, 寻琳婷, 赵雪茹, 张镕

650500 昆明市昆明理工大学医学院（胡建鹏,张镕）; 云南省第一人民医院消化科（宋正己,李玉莲,寻琳婷,赵雪茹）

收稿日期:2017-03-30 出版日期:2018-03-10 发布日期:2018-03-19
通讯作者: 宋正己,E-mail: song4715@163.com
作者简介:胡建鹏,女,25岁,硕士研究生。主要从事慢性肝病细胞分子生物学研究。E-mail:hujianpeng2013_sl@126.com
基金资助:
*国家自然科学基金资助项目（编号：81560107）; 云南省应用基础研究项目（编号：2014FB091）

Expression of Norrin/Frizzled-4 in liver tissues of rats with thioacetamide-induced acute liver injury

Hu Jianpeng, Song Zhengji, Li Yulian, et al

Medical School,Kunming University of Science and Technology,Kunming 650500,Yunnan Province,China

Received:2017-03-30 Online:2018-03-10 Published:2018-03-19

摘要/Abstract

摘要： 目的建立硫代乙酰胺（TAA）诱导的大鼠急性肝损伤模型,探讨Norrin/Frizzled-4在肝损伤间质重建中的作用。方法随机将32只SD大鼠分为正常对照组（N组）8只和TAA诱导组（M组）24只。建立TAA诱导的肝损伤大鼠模型。采用ELISA法检测血清CD105和血管内皮细胞生长因子（VEGF）水平,采用Western blot法检测原代肝星状细胞和肝组织Norrin/Frizzled-4信号通路中细胞外配体蛋白Norrin和细胞膜特异性受体蛋白Frizzled-4的表达。结果 24只TAA诱导组大鼠死亡11只（45.8%）;病理学检查显示急性肝损伤大鼠模型建立成功;对照组血清CD105水平为（2.18±0.05） ng/mL,显著低于TAA处理1 w组的（2.36±0.07） ng/mL或2 w组的（2.42±0.03） ng/mL,差异均有显著性（P<0.05）;对照组血清VEGF为（61.48±0.39） pg/mL,显著低于模型组的（64.52±0.25） pg/mL,差异均有显著性（P<0.01）;原代肝星状细胞和正常肝组织不表达Norrin蛋白或Frizzled-4蛋白,或表达量很低,急性肝损伤大鼠肝组织Norrin/Frizzled-4表达显著高于正常对照组,差异均有显著性（P<0.01）。结论急性肝损伤大鼠肝组织Norrin/Frizzled-4蛋白表达上调,可能与急性肝损伤初期维持血管网络形态及肝间质重建有关。

关键词: 肝损伤, Norrin/Frizzled-4信号通路, 间质重建, 硫代乙酰胺, 大鼠

Abstract: Objective To investigate the roles of Norrin/Frizzled-4 in the interstitial remodeling of acute liver injury. Methods 32 healthy male rats were randomly divided into control（n=8） and model group （n=24）. The model of acute liver injury was established by thioacetamide （TAA） injuction. Western blot was used to detect the expression of Norrin/Frizzled-4 protein in liver tissues,and enzyme-linked immunosorbent assay was performed to measure serum CD105 and vascular endothelial growth factor （VEGF） levels. Results There were 11（45.8%） out of 24 rats died in model group during model establishment. Hematoxylin-eosin staining showed that the acute liver injury model was successfully established. Serum CD105 level was（2.18±0.05） ng/mL and VEGF was （61.48±0.39） pg/mL in control group,significantly lower than[（2.36±0.07） ng/mL and [（62.09±0.54） pg/mL,P<0.05] in TAA-treated for one week,or [2.42±0.03） ng/mL and （64.52±0.25） pg/mL,P<0.05] in TAA-treated for two week;Western blot analysis showed that the expressions of Norrin/Frizzled-4 proteins in model group were higher than in normal control group or in primary hepatic stellate cells（P<0.01）. Conclusion The expressions of Norrin/Frizzled-4 proteins increase in liver tissues of rats with acute liver injury,which might be related to the maintaing of vascular network morphology and interstitial remodeling.

Key words: Acute liver injury, Norrin/Frizzled-4 signaling pathway, Interstitial reconstruction, Thioacetamide, Rats

胡建鹏, 宋正己, 李玉莲, 寻琳婷, 赵雪茹, 张镕. 硫代乙酰胺诱导的急性肝损伤大鼠肝组织Norrin/Frizzled-4表达的变化*[J]. 实用肝脏病杂志, 2018, 21(2): 187-190.

Hu Jianpeng, Song Zhengji, Li Yulian, et al. Expression of Norrin/Frizzled-4 in liver tissues of rats with thioacetamide-induced acute liver injury[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(2): 187-190.

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硫代乙酰胺诱导的急性肝损伤大鼠肝组织Norrin/Frizzled-4表达的变化*

Expression of Norrin/Frizzled-4 in liver tissues of rats with thioacetamide-induced acute liver injury

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