实用肝脏病杂志 ›› 2020, Vol. 23 ›› Issue (2): 256-259.doi: 10.3969/j.issn.1672-5069.2020.02.027

• 肝硬化 • 上一篇    下一篇

乙型肝炎肝硬化患者肠道菌群变化研究*

邢乐康, 邓永东, 姚立琼, 金凤玲   

  1. 730000 兰州市 兰州大学第一临床医学院(邢乐康);第一医院感染病科(邓永东);检验科(姚立琼);感染管理科(金凤玲)
  • 收稿日期:2019-08-26 出版日期:2020-03-10 发布日期:2020-04-20
  • 通讯作者: 金凤玲,E-mail:jfljhk@163.com
  • 作者简介:邢乐康,女,24岁,硕士研究生。研究方向:临床微生物检验
  • 基金资助:
    甘肃省卫生行业科研计划基金资助项目(编号:GSWSKY2017-40)

Changes of intestinal flora in patients with hepatitis B liver cirrhosis

Xing Lekang, Deng Yongdong, Yao Liqiong, et al   

  1. First Clinical Medical College, Lanzhou University, Lanzhou 730000,Gansu Province, China
  • Received:2019-08-26 Online:2020-03-10 Published:2020-04-20

摘要: 目的 分析乙型肝炎肝硬化患者肠道菌群的分布情况及其临床意义。方法 2018年 7月~2019年4月我院收治的乙型肝炎肝硬化患者103例(代偿期肝硬化41例和失代偿期肝硬化62例)和同期健康人群20例,采集粪便,采用Illumina Miseq测序法分析肠道菌群结构差异和物种多样性。结果 在健康人,巨单胞菌属、拟杆菌属和粪杆菌属分别为14.8%、11.4%和9.8%,在代偿期肝硬化组,拟杆菌属、粪杆菌属和巨单胞菌属比例分别为15.2%、11.5%和11.2%,在失代偿期肝硬化组,拟杆菌属、粪杆菌属和韦荣球菌属比例分别为16.9%、9.6%和9.2%;健康人Chao指数为(1250.5±283.7),显著高于代偿期肝硬化组【(1111.5±277.1),P<0.05】或失代偿期肝硬化组【(910.2±369.6),P<0.05】,健康人Shannon指数为(3.7±0.6),显著高于代偿期肝硬化组【(3.5±0.1),P<0.05】或失代偿期肝硬化组【(3.3±0.4),P<0.05】,健康人Simpson指数为(0.3±0.1),显著高于代偿期肝硬化组【(0.2±0.1),P<0.05】或失代偿期肝硬化组【(0.1±0.4),P<0.05】。结论 乙型肝炎肝硬化患者肠道菌群丰度和多样性降低,失代偿期肝硬化患者肠道菌群变化更为明显,即存在随着病情进展肠道菌群多样性减少现象,其临床意义值得探讨。

关键词: 肝硬化, 肠道菌群, 物种丰度

Abstract: Objective The aim of this study was to analyze the distribution of intestinal flora in patients with hepatitis B liver cirrhosis. Methods A total of 103 patients with hepatitis B liver cirrhosis (compensated cirrhosis in 41 and decompensated cirrhosis in 62) and 20 healthy persons were enrolled in our hospital between July 2018 and April 2019, and the sequences of intestinal bacteria were carried out by Illumina Miseq. Results At the genius level, the proportion of Megamonas, Bacteroides and Faecalibacterium in the healthy persons were 14.8%, 11.4% and 9.8%, the proportion of Bacteroides, Faecalibacterium and Megamonas in patients with compensated cirrhosis were 15.2%, 11.5% and 11.2%, while the proportion of Bacteroides, Faecalibacterium and Veillonella in patients with decompensated liver cirrhosis were 16.9%, 9.6% and 9.2%, respectively; we used Chao, Shannon and Simpson index to analyze the flora abundance and diversity, and the Chao index in healthy persons was (1250.5 ±283.7), significantly higher than (1111.5±277.1,P<0.05) in patients with compensated or (910.2 ±369.6, P<0.05) in decompensated liver cirrhosis, the Shannon index in healthy persons was (3.7±0.6), significantly higher than (3.5 ±0.1,P<0.05) in patients with compensated or (3.3±0.4,P<0.05) in decompensated liver cirrhosis, and the Simpson index in the healthy persons was (0.3 ± 0.1), significantly higher than (0.2±0.1, P<0.05) in patients with compensated or (0.1±0.4, P<0.05) in patients with decompensated liver cirrhosis. Conclusion The intestinal flora abundance and diversity in patients with hepatitis B liver cirrhosis decrease as the progress of the disease, which might have a significant implications in dealing with the patients in clinical practice.

Key words: Liver cirrhosis, Intestinal flora, Species abundance