慢性乙型肝炎患者外周血NK细胞杀伤细胞凝集素样受体G1表达及其对NK细胞杀伤功能的影响*

doi:10.3969/j.issn.1672-5069.2020.02.007

摘要/Abstract

摘要： 目的探讨CHB患者外周血NK细胞杀伤细胞凝集素样受体亚家族G成员1(KLRG1)表达的变化以及其对NK细胞杀伤功能的影响。方法在120例CHB患者和120例健康体检者,抽取外周血,提取外周血单个核细胞,使用流式细胞仪检测NK细胞、KLRG1⁺ NK细胞和分泌γ-干扰素的NK细胞百分率,体外检测NK细胞对LX-2细胞的杀伤力。结果 CHB患者外周血NK细胞百分率为(17.2±7.7)%,显著高于对照组 [(11.3±6.9)%,t=9.85,P=0.01】, KLRG1⁺NK细胞百分率为(52.2±20.3)%,显著高于对照组【(30.3±16.9)%,t=6.57,P=0.01】,分泌IFN-γ的NK细胞百分率为(14.6±3.8)%,显著低于对照组【(42.5±9.5)%,t=11.24,P=0.01】;CHB患者外周血NK细胞CD38、CD69、HLA-DR和TRAIL表达均显著高于对照组(P=0.012,P=0.015,P=0.025,P=0.019);CHB患者NK细胞诱导LX-2细胞发生早期凋亡率为11.5% (6.6%～13.7%),晚期凋亡率为7.2% (5.1%～8.5%),与对照组的15.4% (11.5%～24.3%)和13.5% (8.1%～20.4%) 比,显著降低 (U=6.50, P=0.025;U=2.02,P=0.002) 。结论 CHB患者NK细胞表达KLRG1增强,通过减少分泌INF-γ而导致其杀伤功能减弱,可能系造成慢性感染的重要原因。

关键词: 乙型肝炎, 自然杀伤细胞, 杀伤细胞凝集素样受体G1, 杀伤功能

Abstract: Objective The aim of this study was to investigate the expression of lectin-like receptor G1 (KLRG1) in natural killer cells of patients with chronic hepatitis B (CHB). Methods 120 patients with CHB and 120 healthy persons were included in this study, and the percentage of NK cells, KLRG1⁺ NK cells and interferon-γ secreting NK cells from peripheral blood mononuclear cells (PBMCs) were detected by flow cytometry, and the cytotoxicity of NK cells to LX-2 cells was also assayed. Results The percentage of NK cells in peripheral blood of patients with CHB was (17.2±7.7)%, significantly higher than [(11.3±6.9)%, t=9.85, P=0.01], the percentage of KLRG1⁺NK cells was (52.2±20.3)%, much higher than [ (30.3 ±16.9)%, t=6.57, P=0.01] and the percentage of interferon-γ secreting NK cells was(14.6±3.8)%, significantly lower than 【(42.5±9.5)%, t=11.24,P=0.01】 in the control; the expression of CD38, CD69, HLA-DR and TRAIL in NK cells in patients with CHB intensified greatly as compared to those in control (P=0.012, P=0.015, P=0.025, P=0.019);the early apoptosis rate of LX-2 cells in patients with CHB was 11.5% (6.6%-13.7%), and the late apoptosis rate was 7.2% (5.1%-8.5%), decreased greatly as compared to 15.4% (11.5%-24.3%) or 13.5% (8.1%-20.4%) in the control (U=6.50, P=0.025; U=2.02,P=0.002) . Conclusion The expression of KLRG1 in NK cells in patients with CHB intensifies, and the reduced secretion of INF-gamma might decrease the immune functions and lead to chronic infections.

Key words: Hepatitis B, Natural killer cells, Killer cell Lectin-like receptor subfamily G member, Cytotoxicity

殷智颖, 张野, 徐土炳. 慢性乙型肝炎患者外周血NK细胞杀伤细胞凝集素样受体G1表达及其对NK细胞杀伤功能的影响^*[J]. 实用肝脏病杂志, 2020, 23(2): 175-178.

Yin Zhiying, Zhang Ye, Xu Tubing. Intensified expression of lectin-like receptor G1 in natural killer cells of patients with chronic hepatitis B[J]. Journal of Practical Hepatology, 2020, 23(2): 175-178.

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慢性乙型肝炎患者外周血NK细胞杀伤细胞凝集素样受体G1表达及其对NK细胞杀伤功能的影响^*

Intensified expression of lectin-like receptor G1 in natural killer cells of patients with chronic hepatitis B

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