人脐带间充质干细胞移植对酒精性肝损伤大鼠的保护作用*

doi:10.3969/j.issn.1672-5069.2019.06.009

摘要/Abstract

摘要： 目的探讨人脐带间充质干细胞（UCMSCs）移植对酒精性肝损伤大鼠的保护作用。方法将40只SD大鼠随机分为4组,采用白酒灌胃法建立慢性酒精性肝损伤模型,给予UCMSCs移植或UCMSCs联合甘草酸经门静脉移植,在对照组和模型组给予生理盐水处理。检测血清丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）水平。采用RT-PCR法检测肝组织细胞色素P450亚酶（CYP2B1） mRNA水平。结果模型组动物血清AST、ALT和MDA水平分别为（210.6±26.0） U/L、（111.2±13.7） U/L和（1.6±0.1）μmo/L,显著高于对照组的【（85.5±12.7） U/L、（30.8±4.5） U/L和（0.6±0.1） μmo/L,P<0.05】,而血清SOD和GPx水平分别为（141.3±6.9）U/L和（336.5±26.1） U/L,显著低于对照组的【（225.7±16.4） U/L和（912.3±29.5） U/L,P<0.05】;细胞移植或细胞移植联合甘氨酸处理组动物血清酶学变化得到很大的改善;与对照组比,模型组肝组织CYP2B1 mRNA水平显著降低;与模型组和细胞移植组比,细胞移植联合甘氨酸处理组肝组织CYP2B1 mRNA水平显著增强,差异有显著性统计学意义（P<0.05）。结论脐带间充质干细胞移植对大鼠酒精性肝损伤有一定的保护作用,与甘草酸联合应用后,保护效果更加明显。

关键词: 酒精性肝损伤, 脐带间充质干细胞, 移植, 甘草酸, 大鼠

Abstract: Objective To investigate the protective effect of human umbilical cord mesenchymal stem cell （UCMSC） transplantation on alcoholic liver injury in rats. Methods 40 healthy SD rats were randomly divided into control （group A）,model （group B）,UCMSC transplantation （group C） and UCMSC as well as glycyrrhizic acid（group D） intervention. Serum levels of aspartate aminotransferase（AST）,alanine aminotransferase （ALT）,malondialdehyde （MDA）,superoxide dismutase （SOD） and glutathione peroxidase （GPx） were measured and liver tissue cytochrome P450（CYP2B1） mRNA was detected by RT-PCR. Results Serum AST,ALT and MDA levels in the model were （210.6±26.0） U/L,（111.2±13.7） U/L and （1.6±0.1） μmo/L,significantly higher than 【（85.5±12.7） U/L,（30.8±4.5） U/L and（0.6±0.1） μmo/L,respectively,P<0.05】 in the control,while serum levels of SOD and GPx were （141.3±6.9） U/L and （336.5±26.1） U/L,significantly lower than 【（225.7±16.4） U/L and （912.3±29.5） U/L,P<0.05】 in the control;the liver injuries reflected by serum parameter changes improved in rats with UCMSCs transplantation or UCMSCs and glycyrrhizic acid intervention;the CYP2B1 mRNA level in liver tissues in model decreased obviously as compared to that in the control,while they intensified heavily in liver tissues in rats with cell transplantation or in rats with cell and glycyrrhizic acid intervention（P<0.05）. Conclusion Umbilical cord mesenchymal stem cell transplantation might protect alcoholic liver injury in rats to a certain extent,and the combination of glycyrrhizic acid with cell transplantation might improve the protective effect.

Key words: Alcoholic liver injury, Umbilical cord mesenchymal stem cells, Transplantation, Glycyrrhizic acid, Rats

高磊, 曹蕾, 王瑞芳, 冯丹丹, 薛娟. 人脐带间充质干细胞移植对酒精性肝损伤大鼠的保护作用^*[J]. 实用肝脏病杂志, 2019, 22(6): 812-815.

Gao Lei, Cao Lei, Wang Ruifang, et al.. Protective effect of human umbilical cord mesenchymal stem cell transplantation on alcoholic liver injury in rats[J]. Journal of Practical Hepatology, 2019, 22(6): 812-815.

参考文献

[1] 孙文静,刘凯军,文良志,等. 骨髓间充质干细胞对肝脏的影响. 实用肝脏病杂志,2017,20(4):394-396.
[2] Monguió-Tortajada M,Santiago R,Gálvez-Montón C,et al.Nanosized UCMSC-derived extracellular vesicles but not conditioned medium exclusively inhibit the inflammatory response of stimulated T cells:implications for nanomedicine. Theranostics,2017,7(2):270-284.
[3] Campard D,Lysy PA,Najimi M,et al.Native umbilical cord matrix stem cells express hepatic markers and differentiate into hepatocyte-like cells. Gastroenterology,2008,134(3):833-848.
[4] Wang L,Ott L,Seshareddy K,et al.Musculoskeletal tissue engineering with human umbilical cord mesenchymal stromal cells. Regen Med,2011,6(1):95-109.
[5] Jing J,Teschke R.Traditional Chinese medicine and herb-induced liver injury:comparison with drug-induced liver injury. J Clin Transl Hepato,2018,6(1):57-68.
[6] Pingxi W,Fanwei Z,Lina H,et al.Alteration of the immune status of umbilical cord mesenchymal stem cells stimulated by TLR1/2 agonist,Pam3Csk. Mol Med Rep,2016,14(3):2206-2212.
[7] Choi JS,Yoon TJ,Kang KR,et al.Glycoprotein isolated from acanthopanax senticosus protects against hepatotoxicity induced by acute and chronic alcohol treatment. Biol Pharm Bull,2016,29(2):304-306.
[8] Yokoyama A,Kamada Y,Imazeki H,et al.Effects of ADH1B and ALDH2 genetic polymorphisms on alcohol elimination rates and salivary acetaldehyde levels in intoxicated Japanese alcoholic men. Alcohol Clin Exp Res,2016,40(6):1241-1250.
[9] Boye A,Zou YH,Yang Y.Metabolic derivatives of alcohol and the molecular culprits of fibro-hepatocarcinogenesis:Allies or enemies. World J Gastroenterol,2016,22(1):50-71.
[10] Gao B,Xu MJ,Bertola A,et al.Animal models of alcoholic liver disease:Pathogenesis and clinical relevance. Gene Expr,2017,17(3):173-186.
[11] Malinowska K.The role of Cat-262C/T,GPX1 Pro198Leu and Sod1+35A/C gene polymorphisms in a development of primary open-angle glaucoma in a Polish population. Pol J Pathol,2016,67(4):404-410.
[12] Alharby H F,Metwali E M R,Fuller M P,et al. The alteration of mRNA expression of SOD and GPX genes,and proteins in tomato(Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles. Saudi J Biol Sci,2016,23(6):773-781.
[13] Dajovi V.Application of micropatterned co-cultured hepatocytes to evaluate the inductive potential and degradation rate of major xenobiotic metabolizing enzymes. Drug Metab Dispos,2016,44(2):250-261.
[14] Chai NL,Zhang XB,Chen SW,et al.Umbilical cord-derived mesenchymal stem cells alleviate liver fibrosis in rats. World J Gastroenterol,2016,22(26):6036-6048.
[15] Elfayomy AK,Almasry SM,El-Tarhouny SA,et al.Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure:Possible direct and indirect effects. Tissu Cell,2016,48(4):370-382.
[16] Liu FB,Lin Q,Liu ZW.A study on the role of apoptotic human umbilical cord mesenchymal stem cells in bleomycin-induced acute lung injury in rat models. Eur Rev Med Pharmacol Sci,2016,20(5):969-982.
[17] Chung H,Kim Y,Kim HE,et al.Human umbilical cord blood mononuclear cells and chorionic plate-derived mesenchymal stem cells promote axon survival in a rat model of optic nerve crush injury. Int J Mol Med,2016,37(5):1170-1180.
[18] Dong HJ,Shang CZ,Li G,et al.The distribution of transplanted umbilical cord mesenchymal stem cells in large blood vessel of experimental design with traumatic brain injury. J Craniofac Surg,2017,28(6):1615-1619.
[19] Wang LL,Yu Y,Guan HB,et al.Effect of human umbilical cord mesenchymal stem cell transplantation in a rat model of preeclampsia. Reprod Sci,2016,23(8):1058-1070.
[20] Zhou X,Cui L,Zhou X,et al.Induction of hepatocyt-like cells from human umbilical cord-derived mesenchymal stem cells by defined microRNAs. J Cell Mol Med,2017,21(5):881-893.

人脐带间充质干细胞移植对酒精性肝损伤大鼠的保护作用^*

Protective effect of human umbilical cord mesenchymal stem cell transplantation on alcoholic liver injury in rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	徐飞, 李伟荣. 黄芪甲苷对CCL₄所致肝损伤大鼠的保护作用^*[J]. 实用肝脏病杂志, 2019, 22(6): 808-811.
[2]	朱娅鸽, 刘锦峰. 丁二磺酸腺苷蛋氨酸联合复方甘草酸苷治疗酒精性肝硬化患者疗效及其对血清BGP水平的影响^*[J]. 实用肝脏病杂志, 2019, 22(6): 872-875.
[3]	林秋香, 王雪雯, 黄祖雄, 潘晨. 灯盏花素对肝脏缺血再灌注损伤大鼠肝脏和肠黏膜屏障功能的保护作用*[J]. 实用肝脏病杂志, 2019, 22(5): 636-639.
[4]	石小龙, 杜睿, 秦革萍, 薛华燕, 张宏鑫. 七氟醚对脓毒症大鼠肝损伤的保护作用及其作用机制研究*[J]. 实用肝脏病杂志, 2019, 22(5): 640-643.
[5]	赵芳芳, 翟永贞, 冯国和. 自体外周血干细胞移植治疗乙型肝炎肝硬化患者疗效及安全性分析[J]. 实用肝脏病杂志, 2019, 22(5): 700-703.
[6]	王艳, 苏峰, 李园园, 李舒. 蜂毒溶血肽改善非酒精性脂肪肝大鼠肝组织脂肪沉积作用及其机制研究^*[J]. 实用肝脏病杂志, 2019, 22(4): 474-477.
[7]	卢西梅, 康燕飞, 宋智勇, 朱乔. 彩色多普勒超声检查诊断肝移植术后胆道并发症价值分析^*[J]. 实用肝脏病杂志, 2019, 22(4): 589-592.
[8]	周丽云, 李校天, 李丽, 杨俊超. 1,25（OH）₂D₃通过调节miR-146a水平抑制大鼠肝纤维化的体内和体外观察[J]. 实用肝脏病杂志, 2019, 22(3): 333-336.
[9]	周炎仪, 阳灿. 雷公藤多甙片联合异甘草酸镁治疗自身免疫性肝炎患者疗效初步研究[J]. 实用肝脏病杂志, 2019, 22(3): 365-368.
[10]	郭丽平, 张成宏, 宁宝烁, 许敏, 徐贝贝, 王雪, 李异玲. 一种新生大鼠肝细胞分离改良方法的建立^*[J]. 实用肝脏病杂志, 2019, 22(1): 17-20.
[11]	玉苏甫·吐尔逊, 赵燕霞. 小剂量二乙基亚硝胺诱导大鼠肝纤维化模型的建立[J]. 实用肝脏病杂志, 2019, 22(1): 33-36.
[12]	闫焕杰, 张淑芹, 赵文静. 自体骨髓干细胞移植治疗慢加急性乙型肝炎肝衰竭患者疗效初步研究[J]. 实用肝脏病杂志, 2019, 22(1): 81-84.
[13]	王禅, 秦伟, 戴勇, 李晓峰, 张成强. 自体外周血干细胞移植治疗乙型肝炎肝硬化患者临床疗效及其对外周血T淋巴细胞亚群的影响[J]. 实用肝脏病杂志, 2019, 22(1): 97-100.
[14]	刘恩强, 陈果, 廖修用, 许美凤, 魏贵玉, 罗小平. 虾青素对肝纤维化大鼠的保护作用及机制研究^*[J]. 实用肝脏病杂志, 2018, 21(6): 842-846.
[15]	丁蕾, 张振, 刘福全. 实时超声弹性成像技术对原位肝移植患者术后缺血型胆道病变临床评估价值分析^*[J]. 实用肝脏病杂志, 2018, 21(6): 963-966.