自身γδT细胞的体外培养鉴定和治疗慢性乙型肝炎的临床观察

实用肝脏病杂志 ›› 2009, Vol. 12 ›› Issue (6): 420-423.

自身γδT细胞的体外培养鉴定和治疗慢性乙型肝炎的临床观察

刘军权, 陈复兴, 朱云, 王红旗, 张颂, 孙蕾清, 陈桂林, 吕小婷, 张娟, 周忠海

221004江苏省徐州市解放军第97医院实验科（刘军权,陈复兴,张颂,孙蕾清,陈桂林,吕小婷,张娟,周忠海）; 消化内科（朱云）; 感染病科（王红旗）

收稿日期:2009-03-09 出版日期:2009-12-10 发布日期:2016-05-11
作者简介:刘军权男,49岁,副主任技师。主要从事肿瘤和病毒性疾病的细胞免疫治疗。E-mail：xzljq19600115@sina.com

The analysis of therapeutic effect of primary liver cancer treated by stereotactic radiotherapy

LIU Junquan, CHEN Fuxing, ZHU Yun, et al.

Department of Central Laboratory,97 th Hospital of PLA,Xuzhou 221004,P R.China

Received:2009-03-09 Online:2009-12-10 Published:2016-05-11

摘要/Abstract

摘要： 目的探讨采用异戊烯焦磷酸（IPP）刺激人外周血单个核细胞（PBMCs）特异性扩增γ δT细胞,观察其在体外对HBV复制和HBeAg表达的影响,并评估应用γδT细胞过继回输治疗乙型肝炎的疗效。方法在体外应用IPP和IL-2刺激培养人外周血PBMCs8天,采用流式细胞术检测γδT细胞百分率及细胞表面穿孔素、粒酶B、NKG2D和CD44表达量;采用ELISA法检测培养上清IFN-γ、TNF-α和IL-12含量;将γδT细胞与HepG2215细胞按比例共孵育后,检测上清HBeAg和HBV DNA水平。将培养成功的γδT细胞回输治疗慢性乙型肝炎患者50例,12个月后观察疗效。结果人外周血PBMCs在培养前γδT细胞数占5.12%,CD44表达5.13%。在培养8天后则分别升至91.27%和94.00%;γδT细胞表面穿孔素、粒酶B、NKG2D受体表达量（分别为57.1%、71.6%和71.7%）明显高于培养前（分别为0.2%、1.1%和1.3%）;γδT细胞分泌IFN-γ和IL-12量明显增加;扩增的γδT细胞能够抑制HepG2215细胞HBV DNA复制和HBeAg的表达;γδT细胞治疗慢性乙型肝炎患者,HBeAg转阴率为54.3%（25/46）,HBV DNA转阴率为66.0%（33/50）。结论乙型肝炎患者PBMC经刺激培养获得的γδT细胞免疫功能增强,初步观察表明有一定的临床治疗作用。

关键词: 慢性乙型肝炎, γ, δ, T细胞, HepG2215细胞, 免疫治疗

刘军权,陈复兴,朱云,王红旗,张颂,孙蕾清,陈桂林,吕小婷,张娟,周忠海. 自身γδT细胞的体外培养鉴定和治疗慢性乙型肝炎的临床观察[J]. 实用肝脏病杂志, 2009, 12(6): 420-423.

LIU Junquan, CHEN Fuxing, ZHU Yun, et al.. The analysis of therapeutic effect of primary liver cancer treated by stereotactic radiotherapy[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2009, 12(6): 420-423.

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