肝细胞癌患者IL-18基因-137G/C和-607A/C位点单核苷酸多态性变化及其意义探讨*

doi:10.3969/j.issn.1672-5069.2018.06.025

摘要/Abstract

摘要： 目的探讨肝细胞癌（HCC）患者白细胞介素-18（IL-18）基因-137G/C和-607A/C位点单核苷酸多态性（SNP）的变化。方法在178例伴有HBV感染的HCC患者和251例健康人,取外周静脉血提取DNA,采用聚合酶链反应-连接酶检测反应（PCR-LDR）行基因分型,测定IL-18基因-137G/C和-607A/C位点SNP。结果 HCC患者和健康人IL-18-37 G/C基因GG基因型、GC基因型和CC基因型分布频率分别为75.3%对47.0%（P<0.05）、20.8%对51.4%（P<0.05）和3.9%对1.6%（P>0.05）,G等位基因和C等位基因分布频率分别为93.6%对72.7%（P<0.05）和6.4%对27.3%（P<0.05）;HCC患者和健康人IL-18-607A/C位点AA基因型、AC基因型和CC基因型分布频率分别为37.6%对13.5%（P<0.05）、43.3 %对66.9%（P<0.05）和19.1%对19.5%（P>0.05）,A等位基因和C等位基因分布频率分别59.3%对47.0%（P<0.05）和40.7%对53.0%（P<0.05）;HCC组IL-18-137G/C位点的GG基因和G等位基因频率显著高于健康人（P<0.05）,HCC组IL-18-607A/C位点的AA基因和A等位基因频率也显著高于健康人（P<0.05）,提示携带IL-18-137G/C和IL-18-607A/C位点基因型和A等位基因者罹患HCC的风险增加。结论携带IL-18基因-137G/C位点GG基因型和G等位基因以及-607A/C位点AA基因型和A等位基因者可能更容易发生HCC,对HBV感染者筛查这些基因可能有助于早期发现肝肿瘤,以利于早期处理和改善预后。

关键词: 肝细胞癌, 白细胞介素18基因, 单核苷酸多态性

Abstract: Objective To investigate the single nucleotide polymorphisms （SNP） of blood IL-18 gene-137G/C and -607A/C in patients with hepatocellular carcinoma（HCC）. Methods A total of 178 patients with HBV-related HCC and 251 healthy persons were recruited in this study,and peripheral blood were obtained for SNP detection of IL-18 gene-137G/C and -607A/C site by polymerase chain reaction-ligase detection reaction （PCR-LDR）. Results The frequencies of IL-18-37 G/C gene GG,GC and CC genotypes in patients with HCC and healthy persons were 75.3% vs. 47.0% （P<0.05）,20.8% vs. 51.4% （P<0.05） and 3.9% vs. 1.6% （P>0.05）,and the frequencies of G and C alleles were 93.6% vs. 72.7% （P<0.05） and 6.4% vs. 27.3% （P<0.05）,respectively;the frequencies of IL-18-607A/C gene AA, AC and CC genotypes in the two groups were 37.6% vs. 13.5% （P<0.05）,43.3 % vs. 66.9% （P<0.05） and 19.1% vs. 19.5% （P>0.05）,and the frequencies of A and C alleles were 59.3% vs. 47.0% （P<0.05） and 40.7% vs. 53.0% （P<0.05）;the frequency distributions of IL-18-137G/C site GG genotype and G allele in patients with HCC were significantly higher than in healthy persons （P<0.05）,and the frequencies of IL-18-607A/C site AA genotype and A allele in patients with HCC were also significantly higher than in the control（P<0.05）. Conclusion Individuals carrying IL-18-137G/C and IL-18-607A/C gene might be at the risk of HCC susceptible,especially in those with underlying HBV infection, and screening the specific gene might help find out the patients with HCC early in clinical practice.

Key words: Hepatoma, Interleukin-18 gene, Single nucleotide polymorphism

刘晶, 段炜, 张进生, 杨海宁, 孙鹃, 惠增骞. 肝细胞癌患者IL-18基因-137G/C和-607A/C位点单核苷酸多态性变化及其意义探讨^*[J]. 实用肝脏病杂志, 2018, 21(6): 928-931.

Liu Jing, Duan Wei, Zhang Jingsheng, et al.. Single nucleotide polymorphisms of blood IL-18 gene-137G/C and -607A/C in patients with hepatocellular carcinoma[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(6): 928-931.

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肝细胞癌患者IL-18基因-137G/C和-607A/C位点单核苷酸多态性变化及其意义探讨^*

Single nucleotide polymorphisms of blood IL-18 gene-137G/C and -607A/C in patients with hepatocellular carcinoma

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