HGF及其受体c-met在肝衰竭与部分肝切除模型中表达差异性研究*

doi:10.3969/j.issn.1672-5069.2011.06.001

实用肝脏病杂志 ›› 2011, Vol. 14 ›› Issue (6): 401-403.doi: 10.3969/j.issn.1672-5069.2011.06.001

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HGF及其受体c-met在肝衰竭与部分肝切除模型中表达差异性研究^*

朱传龙,李毓雯,李文庭,吴晓玲,高人焘

230001 合肥市安徽医科大学附属省立医院感染病科（朱传龙，李文庭，吴晓玲，高人焘）；儿科（李毓雯）

收稿日期:2011-08-12 出版日期:2011-12-15 发布日期:2016-04-14
通讯作者: 高人焘，E-mail:rentaogao@sina.com
作者简介:朱传龙男，31岁，医学博士，副主任医师。主要从事肝衰竭与肝细胞再生机制研究。
基金资助:
国家自然科学基金（No.30800973）；安徽省优秀青年基金（No.10040606Y14）

Hepatic growth foctor and its receptor c-met expressions in rat models with liver failure and partial hepatectomy

ZHU Chuanlong，LI Yuwen，LI Wenting，et al.

Department of Infectious Disease，Affiliated Provincial Hospital，Anhui Medical University，Hefei 230001，China

Received:2011-08-12 Online:2011-12-15 Published:2016-04-14

摘要/Abstract

摘要： 目的研究HGF及其受体c-met在肝衰竭和部分肝切除两种动物模型肝组织的表达差异。方法分别采用D-GalN/LPS腹腔注射和70%肝脏外科切除术建立肝衰竭和部分肝切除模型，同时以健康大鼠作为对照组。采用ELISA法检测血清HGF水平，采用Real-time PCR和Western-blot法分别检测肝组织c-met基因和蛋白的表达水平。结果与对照组比，部分肝切除模型大鼠血清HGF及其受体c-met表达水平均有明显升高（P<0.01），而在肝衰竭模型大鼠血清HGF水平虽有升高，但c-met蛋白表达水平却明显降低（P<0.01）。结论肝细胞c-met表达水平的降低是D-GalN/LPS诱导肝衰竭大鼠肝细胞再生障碍的主要原因。

关键词: 肝衰竭, 肝再生, c-met, 肝细胞生长素

Abstract: Objective To study hepatic growth factor（HGF）and c-met expressions in rat models with liver failure and partial hepatectomy.Methods Liver failure induced by intraperitoneal injection of LPS/D-GalN and by 70% partial hepatectomy was established，and healthy rats served as controls.The level of serum HGF was detected by ELISA assay and c-met gene expression were detected by real-time PCR and Western-bloting.Results Compared with the control group，the serum HGF level and its receptor c-met were significantly elevated in rats with partial hepatectomy，however，the serum HGF level increased with c-met expression significantly reduced in rats with liver failure.Conclusion The compromised hepatic c-met expression might be essential for defects in HGF-mediated liver regeneration in rats with liver failure.

Key words: Liver failure, Liver regeneration, c-met, Hepatic growth factor

朱传龙,李毓雯,李文庭,吴晓玲,高人焘. HGF及其受体c-met在肝衰竭与部分肝切除模型中表达差异性研究^*[J]. 实用肝脏病杂志, 2011, 14(6): 401-403.

ZHU Chuanlong，LI Yuwen，LI Wenting，et al.. Hepatic growth foctor and its receptor c-met expressions in rat models with liver failure and partial hepatectomy[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2011, 14(6): 401-403.

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HGF及其受体c-met在肝衰竭与部分肝切除模型中表达差异性研究^*

Hepatic growth foctor and its receptor c-met expressions in rat models with liver failure and partial hepatectomy

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