急性肝衰竭大鼠胃肠运动改变与肠神经元关系研究*

doi:10.3969/j.issn.1672-5069.2011.01.002

实用肝脏病杂志 ›› 2011, Vol. 14 ›› Issue (1): 3-6.doi: 10.3969/j.issn.1672-5069.2011.01.002

急性肝衰竭大鼠胃肠运动改变与肠神经元关系研究^*

许伟红刘梅吕志武陈煜郑素军刘旭华赵军丁美段钟平

150086 哈尔滨市哈尔滨医科大学附属第二医院消化内科（许伟红，吕志武）；北京首都医科大学附属北京佑安医院人工肝中心（刘梅，陈煜，郑素军，刘旭华，赵军，丁美，段钟平）

收稿日期:2010-04-15 出版日期:2011-02-10 发布日期:2016-04-15
通讯作者: 段钟平，E-mail:duan2517@163.com
作者简介:许伟红女，26岁，硕士研究生。主要从事胃肠动力学研究。E-mail:xuweihong0107@yahoo.com.cn;刘梅女，37岁，医学博士，主治医师。研究方向：肝衰竭和胃肠动力与激素的关系。E-mail：maybelake@126.com
基金资助:
北京市教育委员会科技发展计划项目（KM201010025024）；“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项课题（2008ZX10002-005-3）；国家自然科学基金课题（30800979，30800517）；北京市科技新星项目（2007B055）

The relationship between enteric neuronal and gastrointestinal motility in rats with acute liver failure

XU Weihong，LIU Mei，LV Zhiwu，et al.

Department of Gastroenterology，Second Affiliated Hospital，Haerbin Medical University，Haerbin 100086，China

Received:2010-04-15 Online:2011-02-10 Published:2016-04-15

摘要/Abstract

摘要： 目的观察急性肝衰竭大鼠胃肠消化间期移行性复合运动的变化与肠神经元的关系。方法采用D-氨基半乳糖诱导的急性肝衰竭大鼠模型，使用多道生理记录仪记录正常大鼠和急性肝衰竭大鼠的胃肠消化间期移行性复合运动，采用TUNEL法、5-HT和NSE免疫组化法检测空肠神经元的凋亡情况。结果急性肝衰竭大鼠空肠MMC周期较对照组显著延长（1897.71±815.77对1384.17±449.34S，P<0.05），尤以Ⅱ期延长为主（1870.90±1010.35S对643.04±450.67S，P<0.05），成簇状移行性收缩，Ⅲ期显著缩短（31.41±11.17S对53.11±14.74S，P<0.05）；急性肝衰竭大鼠肠神经元凋亡明显增多，TUNEL染色阳性细胞核固缩或碎裂，呈棕黄色，细胞大小不一；5-HT和NSE免疫组化检测发现急性肝衰竭大鼠阳性物质表达较正常大鼠减少。结论急性肝衰竭大鼠胃肠MMCⅡ期延长，Ⅲ期明显缩短，导致胃肠动力异常，可能与肠神经元的凋亡有关。

关键词: 急性肝衰竭, 移行性复合运动, 肠神经元

Abstract: Objective This study was undertaken to explore the relationship between enteric neuronal and migrating motor complexes（MMC）in rats with acute liver failure. Methods Wistar rats were given the D-galactosamine hydrochloride（D-Gal）to induce acute liver failure. Then，the MMC was recorded by a multilead physiologival recording system. TUNEL，5-HT and NSE immunohistochemistry were used to detect neuronal apoptosis. Results The duration of jejunal MMC was significantly prolonged in rats with acute liver failure compared with the control（P<0.05）；The duration of phaseⅡ was also significantly prolonged，and multiple clusters of contractions was observed in phaseⅡ；However，the duration of phaseⅢ was significantly shortened（P<0.05）compared with the control group；TUNEL staining showed neuronal apoptosis increased significantly in rats with acute liver failure；The content of NSE and 5-HT positive substances of jujunal in rats with acute liver failure was markedly decreased. Conclusion The prolonged duration of MMC and phaseⅡ and decreased duration of phaseⅢ might results in abnormal gastrointestinal motility，which may be related to enteric neuronal apoptosis.

Key words: Acute liver failure, Migrating motor complex, Enteric neuronal

许伟红刘梅吕志武陈煜郑素军刘旭华赵军丁美段钟平. 急性肝衰竭大鼠胃肠运动改变与肠神经元关系研究^*[J]. 实用肝脏病杂志, 2011, 14(1): 3-6.

XU Weihong，LIU Mei，LV Zhiwu，et al.. The relationship between enteric neuronal and gastrointestinal motility in rats with acute liver failure[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2011, 14(1): 3-6.

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急性肝衰竭大鼠胃肠运动改变与肠神经元关系研究^*

The relationship between enteric neuronal and gastrointestinal motility in rats with acute liver failure

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