Objective To probe the membrane protein changes in rats with hepatic fibrosis after the treatment of Fufang Biejia Ruangan Pian. Method This research set up an rat model with porcine sera-induced hepatic fibrosis and the model rats were treated by intragastric infusion of Fufang Biejia Ruangan Pian solution for 8 weeks. Plasma membrane was purified by density gradient centrifugation,the purity of them was verified by Western blot and differential proteomic analysis of liver plasma membrane was done. Result An animal model of hepatic fibrosis was set up successfully at week 8. After treatment with Fufang Biejia Ruangan Pian for eight weeks,the livers returned to normal levels;Western blot analysis showed that the plasma membrane was enriched while the contamination of other subcellular fractions such as mitochondrion reduced. 22 proteins with more than three-fold differences were found and 9 proteins were successfully identified. Western blotting verified the different expression of keratin 8 and annexin A2. Conclusion Fufang Biejia Ruangan Pian has anti-fibrotic effects by regulating keratin 8 and annexin A2 expression in the plasma membrane during hepatic fibrosis process.

Objective To explore the effect of apoptosis-regulated proteins Bcl-2 and Bax on the 1iver of rats with nonalcoholic steatohepatitis. Methords 20 male Wistar rats were randomly divided into standard diet-fed group and high fat diet-fed group. After 12 weeks,the levels of serum triglyceride,total cholesterol,ALT and AST were measured. The levels of Bcl-2 and Bax Proteins were determined by Western blot. Results The NASH rats exhibited severe steatohepatitis with scattered punctual necrosis;The relative level of Bax in liver tissue of rats with NASH was significantly higher than that in controls（1.02±0.33 vs.0.51±0.03,P<0.01）,while the expression of Bcl-2 was decreased（0.15±0.31 vs 0.52±0.01,P<0.01）. The Bcl-2/Bax ratio also decreased in the NASH group（0.14±0.01 vs 1.03±0.06,P<0.01）. Conclusion Bcl-2 and Bax proteins could play a role in rats with nonalcoholic steatohepatitis.

Objective To investigate the protective effect of ischemic-postconditioning （IPO） on hepatic ischemia-reperfusion injury in rats. Methods 75 Sprague-Dawley rats were randomly divided into shamoperated（S）,ischemia-reperfusion（IR） and IPO group to establish the model of acute liver ischemia-reperfusion injuries. Before persistent reperfusion,animals in IPO group were given six brief reperfusion-ischemia. The sample of blood and hepatic tissue of rats in all groups were taken for detections. Results Compared with in IR group,the concentrations of serum AST and ALT and malondialdehyde in hepatic tissues in IPO group decreased markedly（P<0.01）,while the concentrations of superoxide dismutase increased significantly（P<0.01）;the hepatic pathological damage also relieved obviously in IPO group. The expression levels of enos protein in IPO group were higher than that of IR group at the 7 min reperfusion. Conclusion IPO can attenuate hepatic ischemia-reperfusion injury by activating the prosurvival kinases of eNOS in accordance with the RISK pathway and reducing the concentrations of oxygen free radical.

Objective To observe the effects of telbivudine combined with oxymatrine in patients with HBeAg positive chronic hepatitis B. Methods 65 cases of HBeAg positive CHB were randomly divided into two groups. In combined group（33 cases）,telbivudine was given 600 mg once a day and oxymatrine 0.6g/d. In telbivudine group（32 cases）,telbivudine was given alone. Clinical evaluation and laboratory detection were performed at the 12,26,52 weeks of the follow up period. Results At 12 weeks,undetectable rates of serum HBV DNA in combination group and telbivudine group were 45.4% and 31.3% respectively（P>0.05）;at 26 weeks,the rates were 72.7% and 46.9%,respectively（P<0.05）;at 52 weeks,the rates were 81.8% and 59.4%,respectively（P<0.05）. Seroconversion rates of HBeAg/HBeAb in combination group and telbivudine group were 12.1% and 6.3% respectively at 12 weeks（P>0.05）,But the seroconversion rates were 30.3% and 9.38% respectively at 26 weeks（P<0.05）; the seroconversion rates were 45.5% and 21.9% respectively at 52 weeks（P<0.05）. The normalization rates of ALT and TBIL in the two groups had no statistically significant difference（P>0.05）. There were no severe adverse reaction in both groups. Conclusion Telbivudine combined with Oxymatrine could enhance the effect of antivirus therapy for patients with chronic hepatitis B.

Objective To investigate the changes of blood gas analysis in patients with chronic liver failure and decompensated cirrhosis. Methods The blood gas analysis in 37 patients with chronic liver failure and decompensated cirrhosis were analyzed. Results Two patients had hypoxia,four patients had orthostatic hypoxia and 11 patients had mild chest tightness. 36 patients had acid-base imbalance in chronic liver failure group,nine had a simple,ten double and two triple acid-base imbalance;in decompensated cirrhosis group,eight had simple and 7 double acid-base imbalance;alkalosis-based type of acid-base imbalance was common in both of the two groups. In chronic liver failure group,the three major types were respiratory alkalosis with metabolic acidosis（38.1%）,respiratory alkalosis（23.8%）,and metabolic alkalosis（14.3%）. In decompensated cirrhosis group,the three major types were respiratory alkalosis（46.7%）,respiratory alkalosis with metabolic alkalosis（26.7%）,and respiratory alkalosis with metabolic acidosis（20.0%）. Conclusion Chronic liver failure and decompensated cirrhosis patients have acid-base imbalance and hypoxemia. Dynamic monitoring of blood gas might have a clinical significance.

Objective To study the therapeutic effects of transcatheter arterial chemoembolization combined with lienalis infusion indirectly to portal vein in treating patients with primary liver cancer. Methods 30 patients with PLC underwent transcatheter arterial chemoembolization treatment,while 40 underwent transcatheter arterial chemoembolization combined with lienalis infusion to portal vein. The serum levels of TNF-α,IL-2,sIL-2R and VEGF were assessed by ELISA. Results The serum levels of TNF-α,sIL-2R as well as the VEGF levels in the two groups decreased（P<0.01）,while IL-2 increased（P<0.01）after treatment;The 1,2 and 3 year survival rates of patients with transcatheter arterial chemoembolization combined with lienalis infusion to portal vein were 72.5%,55% and 25%,higher than that of single TACE therapy group（60%,30% and 6.7%,respectively,P<0.05）. Conclusion Transcatheter arterial chemoembolization combined with lienalis infusion in treating patients with primary liver cancer can improve the immune function and enhance the curative effect.

Objective To analyze the therapeutic effect of primary liver cancer treated by stereotactic radiotherapy. Methods 266 patients with primary liver cancer were treated with γ ray stereotatic radiotherapy（SRT）. The dose of 3200~4600cGy（300~500cGy/F,6F/w） were delivered to PTVs over 7 to 10 fracions,6 consecutive fractions per week. Results All patients were examined with image of CT or MRI. The total effective rate（CR+PR）,steady diseae（SD）,progressive disease（PD） was 79%,18% and 3%. The 1,2,3-year survival rates of patcents were 71.2%,40.3%,22.8%,respectively. No RILD and serious side effect occurred,and the gastrointestinal complications were uncomfortable in upper abdomen and poor appetite. Conclusions Stereotactic radiotherapy has good effect and little side effect in the treatment of primary liver cancer.