Abstract: Objective To investigate the effect of three hepatic vascular occlusion maneuvers on the apoptosis of liver cells during ischemia-reperfusion injury in rats. Methods One hundred and twenty-eight Wistar rats were randomly divided into the sham operation group（SO，n=8），Pringle group（PR，n=40），hemihepatic vascular occlusion group（HH，n=40）and hepatic blood inflow occlusion without hemihepatic artery control group（WH，n=40）. Each group except for the SO group was further devided into 5 subgroups（n=8），which were 1h，2h，6h，24h and 72h group，respectively. After 30min of hepatic vascular occlusion with three methods，the liver blood flow was reperfused. The liver cell apoptosis rate，hepatic histopathology and the survival rate at 72 hours after reperfusion were measured. Results After each period of reperfusion，ALT，AST and apoptosis rates were significantly higher in PR group than in HH and WH group，and the pathological and ultrastructural changes of hepatic tissues at 6 hours after reperfusion were significantly worse in PR group than in HH and WH group；Survival rates at 72 hours after reperfusion were 87.5%（7/8），100%（8/8）and 100%（8/8），respectively，in PR，HH and WH group. Conclusion Hepatic blood inflow occlusion without hemihepatic artery control can reduce hepatic ischemia-reperfusion injury.

Key words: Ischemia reperfusion injuries, Vascular occlusion, Apoptosis, Rats