LINE-1 ORF-1p过表达对肝癌细胞株SMMC7721增殖和锚定非依赖性生长的影响*

doi:10.3969/j.issn.1672-5069.2011.05.002

JOURNAL OF PRACTICAL HEPATOLOGY ›› 2011, Vol. 14 ›› Issue (5): 323-326.doi: 10.3969/j.issn.1672-5069.2011.05.002

Previous Articles Next Articles

Effects of LINE-1 ORF-1p overexpression on proliferation and anchor-independent growth of SMMC7721 hepatoma cell line

GAO Xudong，LU Yinying，FENG Fan，et al.

Center for Liver Cancer，302nd Hospital of PLA，Beijing 100039，China

Received:2011-06-08 Online:2011-10-15 Published:2016-04-14

Abstract

Abstract: Objective To investigate the effects of L1 encoded ORF-1p on the proliferation of SMMC7721 cells. Methods Recombinant expression vector pcDNA3.0-Flag-ORF1 was used to transfect SMMC7721 cells;Expression of ORF1p in the transfected cells was detected by Western blotting;Proliferation and anchor-independent growth of the cells transfected with Flag-ORF1p vector were detected by MTT assays and soft-agar anchored colony formation assays;Transcriptional activity of p15，p21 and p53 reporter genes was analyzed by luciferase reporter system. Results Flag-ORF-1p could enhance the proliferation and anchor-independent growth ability of SMMC7721 cells;It also reduced the transcriptional activity of p15，p21 and p53 luciferase report genes. Conclusion ORF-1p of L1 might enhance the SMMC7721 cell proliferation and anchor-independent growth through regulation of some genes.

Key words: SMMC7721 cells, Retrotransposon LINE-1, Open reading frame-1p, Carcinogenesis

GAO Xudong，LU Yinying，FENG Fan，et al.. Effects of LINE-1 ORF-1p overexpression on proliferation and anchor-independent growth of SMMC7721 hepatoma cell line[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2011, 14(5): 323-326.

References

[1] ROMAN-GOMEZ J，JIMENEZ-VELASCO A，AGIRRE X，et al. Promoter hypomethylation of the LINE-1 retrotransposable elements activates sense/antisense transcription and marks the progression of chronic myeloid leukemia[J]. Oncogene，2005，24:7213-7223.
[2] GOODIER JL，ZHANG L，VETTER MR，et al. LINE-1 ORF1 protein localizes in stress granules with other RNA-binding proteins，including components of RNA interference RNA-induced silencing complex[J]. Mol Cell Biol，2007，27:6469-6483.
[3] PORNTHANAKASEM W，KONGRUTTANACHOK N，PHUANGPHAIROJ C，et al. LINE-1 methylation status of endogenous DNA double-strand breaks[J]. Nucleic Acids Res，2008，36（11）:3667-3675.
[4] GASIOR SL，WAKEMAN TP，XU B，et al. The human LINE-1 retrotransposon creates DNA double-strand breaks[J]. J Mol Biol，2006，357:1383-1393.
[5] XING J，ZHANG Y，HAN K，et al. Mobile elements create structural variation:analysis of a complete human genome[J].Genome Res，2009，19:1516-1526.
[6] ESTECIO MR，GHARIBYAN V，SHEN L，et al. LINE-1 hypomethylation in cancer is highly variable and inversely correlated with microsatellite instability[J]. PLoS ONE，2007，2（5）:e399.
[7] SINIBALDI-VALLEBONA P，LAVIA P，GARACI E，et al.A role for endogenous reverse transcriptase in tumorigenesis and as a target in differentiating cancer therapy[J]. Genes Chromosomes Cancer，2006，45（1）:1-10.
[8] MARTIN SL. The ORF1 protein encoded by LINE-1:structure and function during L1 retrotransposition[J]. J Biomed Biotechnol，2006，2006（1）:45621.
[9] 高旭东，胡明明，朱运峰. 下调LINE-1编码蛋白ORF-1p对肺癌细胞A549生物学特征的影响[J]. 中国生物工程杂志，2010，30（6）：14-20.
[10] BUNZ F，DUTRIAUX A，LENGAUER C，et al. Requirement for p53 and p21 to sustain G2 arrest after DNA damage[J]. Science，1998，282（5393）:1497-1501.
[11] SCOTT MT，MORRICE N，BALL KL. Reversible phosphorylation at the C-terminal regulatory domain of p21（Waf1/Cip1）modulates p roliferating cell nuclear antigen binding[J]. J Biol Chem，2000，275（15）:11529-11537.

Effects of LINE-1 ORF-1p overexpression on proliferation and anchor-independent growth of SMMC7721 hepatoma cell line

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments