Abstract: Objective The purpose of this experiment was to investigate the dynamic changes of blood glucose levels and gut microbiota in mice with high-fat diet-induced metabolic associated fatty liver diseases (MAFLD). Methods Twelve C57BL/6 mice were fed with 60% fat-calorie high-fat diet for 24 weeks. AT 0 weeks, 8 weeks, 16 weeks and 24 weeks, the glucose tolerance test (GTT) and insulin resistance test (ITT) were performed to evaluate glucose metabolism and insulin sensitivity. The mice feces were collected for 16sRNA detection to analyze the changes of bacterial structure and diversity of gut microbiota. Results As the high-fat diet prolonged, the blood glucose by GTT and by ITT increased significantly (P <0.05); the chao1 index and ACE index increased, suggesting that the diversity of intestinal microflora increased as the high-fat diet continued; at the phylum levels, the percentages of Firmicutes and Proteobacteria at week 8, 16 and 24 were significantly increased compared with that at week 0 (P < 0.05), while the percentages of Bacteroidetes and Verrucomicrobia were significantly decreased (P <0.05); at the genus levels, the percentages of Akkermansia and Ruminococcaceae-UCG-014 were significantly decreased after high-fat diet at week 8, 16 and 24 (P<0.05), while percentage of Coriobacteriaceae-UCG_002 was significantly increased (P<0.05) compared with that at week 0; at week 24, the percentage of Dubosiella increased compared with that at week 0 (P<0.05). Conclusion With the prolongation of high-fat diet feeding, the blood glucose levels of mice gradually increase, the alpha diversity of the gut microbiota continues to increase, and the bacterial structure at the phylum and genus levels change, which warrants further investigation.

Key words: Metabolic associated fatty liver disease, High-fat diet, Blood glucose, Gut microbiota, Mice