Abstract: Objective The aim of this preliminary study was to explore the efficacy of laparoscopic hepatic portoenteral anastomosis （HPEA） in the treatment of neonatal with biliary atresia （BA） and the changes of serum interleukin（IL）-12p40 and IL-13Rα2 levels. Methods 72 infants with BA were recruited in our hospital between March 2014 and June 2018,and laparoscopic HPEA was performed in 42,and open HPEA was undergone in 30 infants. Serum IL-12p40 and IL-13Rα2 levels were detected by ELISA. Results At presentation,the ages,heights and body weights in the two groups were not significantly different（P>0.05）;the operation time in the laparoscopic HPEA was longer than that in the open operation [（187.8±32.6） min vs. （152.4±39.3） min,P<0.05],while the intraoperative blood loss and feeding time were much less or shorter than in the open surgery [（15.3±5.2） mL vs.（33.6±12.6） mL,P<0.05 and （1.3±0.4）d vs.（2.3±0.7） d,respectively,P<0.05], and there was no significant difference as respect to the postoperative anesthesia recovery time between the two groups [（77.5±18.0） min vs.（82.1±20.2）min,P>0.05];before operation,serum IL-12p40 and IL-13Rα2 levels in the open operation were [0.7（0.1,2.0）] ng/mL and [6.3（2.2,17.6）] ng/mL,not significantly different as compared to [0.8（0.2,2.4）] ng/mL and [6.7（2.0,19.3）] ng/mL in laparoscopic operation,and after operation,they were [0.4（0.1,0.9）] ng/mL and [1.1（0.4,3.6）] ng/mL,also not significantly different compared to [0.4（0.2,1.0）] ng/mL and [1.1（0.6,4.0）] ng/mL,respectively,in the laparoscopic HPEA （P>0.05）;at three-month follow-up, 37（51.4%） infants with BA survived,and 5（11.9%） laparoscopic HPEA-treated and 4 open HPEA-treated infants died （P>0.05）. Conclusion Laparoscopic HPEA is an alternative modality for the management of neonatal with biliary atresia.

Key words: Biliary atresia, Laparoscopy, Hepatic portoenteral anastomosis, Interleukin-12p40, IL-13Rα2;